BRENDA - Enzyme Database show
show all sequences of 5.1.3.6

The biosynthesis of D-galacturonate in plants. functional cloning and characterization of a membrane-anchored UDP-D-glucuronate 4-epimerase from Arabidopsis

Molhoj, M.; Verma, R.; Reiter, W.D.; Plant Physiol. 135, 1221-1230 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Pichia pastoris
Arabidopsis thaliana
General Stability
General Stability
Organism
freezing and thawing of the crude extract in absence of cryoprotectants abolishes enzymatic activity entirely
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition: UDP, UDP-D-glucose or UDP-D-galactose
Arabidopsis thaliana
UDP-D-Xyl
strong
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
UDP-glucuronate
-
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
exclusively localized in microsomal fraction, anchored to membrane
Arabidopsis thaliana
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O22141
-
-
Arabidopsis thaliana
Q67ZJ4
-
-
Arabidopsis thaliana
Q9M0B6
-
-
Arabidopsis thaliana
Q9STI6
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
strong expression
Arabidopsis thaliana
-
leaf
moderate expression; strong expression
Arabidopsis thaliana
-
additional information
moderate expression throughout the plant; moderate expression throughout the plant; moderate expression throughout the plant; no expression in root or inflorescence
Arabidopsis thaliana
-
root
strong expression
Arabidopsis thaliana
-
silique
moderate expression; strong expression
Arabidopsis thaliana
-
stem
strong expression
Arabidopsis thaliana
-
Storage Stability
Storage Stability
Organism
-20°C or -80°C in 50% v/v glycerol, 7 days, enzyme retains approximately 80% of its activity
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no epimerization of UDP-D-glucose and UDP-D-xylulose
663125
Arabidopsis thaliana
?
-
-
-
-
UDP-glucuronate
-
663125
Arabidopsis thaliana
UDP-D-galacturonate
-
-
-
?
UDP-glucuronate
the recombinant enzyme establishes a 1.3:1 equilibrium between UDP-D-galacturonate and UDP-glucuronate
663125
Arabidopsis thaliana
UDP-D-galacturonate
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
-
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8.9
pH 5.0: about 75% of maximal activity, pH 8.9: 93% of maximal activity
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
GAE1 expressed in Pichia pastoris already contains tightly bound NAD+ or NADP+
Arabidopsis thaliana
NADP+
GAE1 expressed in Pichia pastoris already contains tightly bound NAD+ or NADP+
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Pichia pastoris
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
GAE1 expressed in Pichia pastoris already contains tightly bound NAD+ or NADP+
Arabidopsis thaliana
NADP+
GAE1 expressed in Pichia pastoris already contains tightly bound NAD+ or NADP+
Arabidopsis thaliana
General Stability (protein specific)
General Stability
Organism
freezing and thawing of the crude extract in absence of cryoprotectants abolishes enzymatic activity entirely
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition: UDP, UDP-D-glucose or UDP-D-galactose
Arabidopsis thaliana
UDP-D-Xyl
strong
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
UDP-glucuronate
-
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
exclusively localized in microsomal fraction, anchored to membrane
Arabidopsis thaliana
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
strong expression
Arabidopsis thaliana
-
leaf
strong expression
Arabidopsis thaliana
-
leaf
moderate expression
Arabidopsis thaliana
-
additional information
moderate expression throughout the plant
Arabidopsis thaliana
-
additional information
no expression in root or inflorescence
Arabidopsis thaliana
-
root
strong expression
Arabidopsis thaliana
-
silique
strong expression
Arabidopsis thaliana
-
silique
moderate expression
Arabidopsis thaliana
-
stem
strong expression
Arabidopsis thaliana
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C or -80°C in 50% v/v glycerol, 7 days, enzyme retains approximately 80% of its activity
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no epimerization of UDP-D-glucose and UDP-D-xylulose
663125
Arabidopsis thaliana
?
-
-
-
-
UDP-glucuronate
-
663125
Arabidopsis thaliana
UDP-D-galacturonate
-
-
-
?
UDP-glucuronate
the recombinant enzyme establishes a 1.3:1 equilibrium between UDP-D-galacturonate and UDP-glucuronate
663125
Arabidopsis thaliana
UDP-D-galacturonate
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
-
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8.9
pH 5.0: about 75% of maximal activity, pH 8.9: 93% of maximal activity
Arabidopsis thaliana
Other publictions for EC 5.1.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716581
Wojtasik
The changes in pectin metaboli ...
Linum usitatissimum
Plant Physiol. Biochem.
49
862-872
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
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-
-
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-
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-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
716216
Pang
Comparative proteomics indicat ...
Arabidopsis thaliana, Gossypium hirsutum
Mol. Cell. Proteomics
9
2019-2033
2010
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
-
2
-
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-
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-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
700488
Siddique
Myo-inositol oxygenase genes a ...
Arabidopsis thaliana
New Phytol.
184
457-472
2009
-
-
1
-
-
-
-
-
-
-
-
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-
6
-
-
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-
2
-
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-
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6
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-
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-
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-
-
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-
12
-
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-
-
-
-
-
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-
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-
-
-
-
-
-
-
704357
Gu
Enzymatic characterization and ...
Arabidopsis thaliana, Oryza sativa, Zea mays
J. Biochem.
146
527-534
2009
-
-
3
-
1
-
6
4
-
-
7
5
-
12
-
-
3
-
-
-
-
1
5
3
3
-
3
4
3
1
-
-
10
-
-
-
-
5
-
-
1
-
-
10
10
4
-
-
8
5
-
-
-
5
-
-
-
1
5
4
5
-
3
4
4
1
-
-
-
-
-
-
-
-
661739
Usadel
Identification and characteriz ...
Arabidopsis thaliana
FEBS Lett.
569
327-331
2004
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
8
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
663125
Molhoj
The biosynthesis of D-galactur ...
Arabidopsis thaliana
Plant Physiol.
135
1221-1230
2004
-
-
1
-
-
1
2
1
1
-
-
-
-
4
-
-
-
-
-
6
-
1
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
11
-
1
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
663134
Gu
The biosynthesis of UDP-galact ...
Arabidopsis thaliana
Plant Physiol.
136
4256-4264
2004
-
-
1
-
-
1
5
1
-
-
2
-
-
3
-
-
1
-
-
4
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
5
-
1
-
-
2
-
-
-
-
1
-
4
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
653259
Munoz
First molecular characterizati ...
Streptococcus pneumoniae
Mol. Microbiol.
31
703-713
1999
-
-
1
-
-
-
12
1
1
-
2
1
-
3
-
-
1
-
-
-
-
-
2
1
1
-
4
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
12
-
1
1
-
2
1
-
-
-
1
-
-
-
-
2
1
1
-
4
-
1
1
-
-
-
-
-
-
-
-
2403
Bolwell
-
Decrease of polygalacturonic a ...
Acer pseudoplatanus
Phytochemistry
24
699-702
1985
-
-
-
-
-
-
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-
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
2401
Dalessandro
Changes in enzymic activities ...
Abies grandis, Pinus sylvestris
Biochem. J.
162
281-288
1977
-
-
-
-
-
-
-
-
-
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-
2
-
2
-
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-
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4
-
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4
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2
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4
-
-
4
-
-
-
-
-
-
-
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2405
Maitra
The mechanism of uridine dipho ...
Dolichospermum flos-aquae
J. Biol. Chem.
249
3075-3078
1974
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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2406
Gaunt
Uridine diphosphate galacturon ...
Dolichospermum flos-aquae
J. Biol. Chem.
249
2366-2372
1974
-
-
-
-
-
-
14
1
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1
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2
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1
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1
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1
1
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14
-
1
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1
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1
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1
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1
1
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-
-
-
2407
Davidson
-
UDP-D-galacturonic acid 4-epim ...
Raphanus sativus
Methods Enzymol.
8
276-277
1966
-
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-
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1
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1
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1
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1
1
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1
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1
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1
1
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-
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-
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-
2408
Feingold
The 4-epimerization and decarb ...
Vigna radiata var. radiata
J. Biol. Chem.
235
910-913
1960
-
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1
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1
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