BRENDA - Enzyme Database show
show all sequences of 5.1.3.6

Identification and characterization of a UDP-D-glucuronate 4-epimerase in Arabidopsis

Usadel, B.; Schluter, U.; Molhoj, M.; Gipmans, M.; Verma, R.; Kossmann, J.; Reiter, W.D.; Pauly, M.; FEBS Lett. 569, 327-331 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Pichia pastoris
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
UDP-D-glucuronate
25°C, pH 7.9, recombinant enzyme
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
cv. Columbia-0
-
Arabidopsis thaliana
Q9LIS3
cv. Columbia-0
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
anther
-
Arabidopsis thaliana
-
cauline leaf
; GAE1; GAE2
Arabidopsis thaliana
-
flower
; GAE1; GAE2
Arabidopsis thaliana
-
leaf
GAE1; GAE2; only in leaf veins
Arabidopsis thaliana
-
pollen
only detected after pollen maturation
Arabidopsis thaliana
-
root
expressed in root hair zone; expression of GAE4 is confined to root tips; GAE1 is expressed throughout the root; GAE2 is expressed throughout the root; GAE3 is expressed in root hair zone
Arabidopsis thaliana
-
silique
; GAE1; GAE2
Arabidopsis thaliana
-
stem
; GAE1; GAE2
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucuronate
-
661739
Arabidopsis thaliana
UDP-D-galacturonate
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
-
-
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
pH 5.0: about 65% of maximal activity, pH 9.0: about 95% of maximal activity
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Pichia pastoris
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
UDP-D-glucuronate
25°C, pH 7.9, recombinant enzyme
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
anther
-
Arabidopsis thaliana
-
cauline leaf
-
Arabidopsis thaliana
-
cauline leaf
GAE1; GAE2
Arabidopsis thaliana
-
flower
-
Arabidopsis thaliana
-
flower
GAE1; GAE2
Arabidopsis thaliana
-
leaf
only in leaf veins
Arabidopsis thaliana
-
leaf
GAE1; GAE2
Arabidopsis thaliana
-
pollen
only detected after pollen maturation
Arabidopsis thaliana
-
root
expressed in root hair zone
Arabidopsis thaliana
-
root
expression of GAE4 is confined to root tips; GAE1 is expressed throughout the root; GAE2 is expressed throughout the root; GAE3 is expressed in root hair zone
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
silique
GAE1; GAE2
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
stem
GAE1; GAE2
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucuronate
-
661739
Arabidopsis thaliana
UDP-D-galacturonate
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
-
-
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
pH 5.0: about 65% of maximal activity, pH 9.0: about 95% of maximal activity
Arabidopsis thaliana
Other publictions for EC 5.1.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748715
Yin
cDNA isolation and functional ...
Ornithogalum longebracteatum
Molecules
21
1505
2016
-
-
1
-
1
-
-
-
2
-
-
1
-
2
-
-
-
-
-
5
-
-
1
1
2
1
-
-
2
1
-
1
-
1
-
-
-
1
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
5
-
-
1
1
2
1
-
-
2
1
-
1
1
4
4
1
-
-
716581
Wojtasik
The changes in pectin metaboli ...
Linum usitatissimum
Plant Physiol. Biochem.
49
862-872
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
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1
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1
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1
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1
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-
-
-
1
1
1
1
-
-
716216
Pang
Comparative proteomics indicat ...
Arabidopsis thaliana, Gossypium hirsutum
Mol. Cell. Proteomics
9
2019-2033
2010
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
2
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-
3
-
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2
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2
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-
-
2
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
700488
Siddique
Myo-inositol oxygenase genes a ...
Arabidopsis thaliana
New Phytol.
184
457-472
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
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-
-
2
-
-
-
-
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6
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-
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12
-
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-
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-
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-
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-
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-
-
-
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-
704357
Gu
Enzymatic characterization and ...
Arabidopsis thaliana, Oryza sativa, Zea mays
J. Biochem.
146
527-534
2009
-
-
3
-
1
-
6
4
-
-
7
5
-
12
-
-
3
-
-
-
-
1
5
3
3
-
3
4
3
1
-
-
10
-
-
-
-
5
-
-
1
-
-
10
10
4
-
-
8
5
-
-
-
5
-
-
-
1
5
4
5
-
3
4
4
1
-
-
-
-
-
-
-
-
661739
Usadel
Identification and characteriz ...
Arabidopsis thaliana
FEBS Lett.
569
327-331
2004
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
8
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
663125
Molhoj
The biosynthesis of D-galactur ...
Arabidopsis thaliana
Plant Physiol.
135
1221-1230
2004
-
-
1
-
-
1
2
1
1
-
-
-
-
4
-
-
-
-
-
6
-
1
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
11
-
1
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
663134
Gu
The biosynthesis of UDP-galact ...
Arabidopsis thaliana
Plant Physiol.
136
4256-4264
2004
-
-
1
-
-
1
5
1
-
-
2
-
-
3
-
-
1
-
-
4
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
5
-
1
-
-
2
-
-
-
-
1
-
4
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
653259
Munoz
First molecular characterizati ...
Streptococcus pneumoniae
Mol. Microbiol.
31
703-713
1999
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-
1
-
-
-
12
1
1
-
2
1
-
3
-
-
1
-
-
-
-
-
2
1
1
-
4
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
12
-
1
1
-
2
1
-
-
-
1
-
-
-
-
2
1
1
-
4
-
1
1
-
-
-
-
-
-
-
-
2403
Bolwell
-
Decrease of polygalacturonic a ...
Acer pseudoplatanus
Phytochemistry
24
699-702
1985
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2401
Dalessandro
Changes in enzymic activities ...
Abies grandis, Pinus sylvestris
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281-288
1977
-
-
-
-
-
-
-
-
-
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2
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2
-
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-
-
-
4
-
-
4
-
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2
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-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
2405
Maitra
The mechanism of uridine dipho ...
Dolichospermum flos-aquae
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249
3075-3078
1974
-
-
-
-
-
-
-
-
-
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-
-
1
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1
-
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1
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1
-
-
-
-
-
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-
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2406
Gaunt
Uridine diphosphate galacturon ...
Dolichospermum flos-aquae
J. Biol. Chem.
249
2366-2372
1974
-
-
-
-
-
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14
1
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1
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2
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1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
1
-
-
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-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2407
Davidson
-
UDP-D-galacturonic acid 4-epim ...
Raphanus sativus
Methods Enzymol.
8
276-277
1966
-
-
-
-
-
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1
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1
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1
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1
1
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1
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1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
2408
Feingold
The 4-epimerization and decarb ...
Vigna radiata var. radiata
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235
910-913
1960
-
-
-
-
-
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-
-
-
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1
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1
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1
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1
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