BRENDA - Enzyme Database show
show all sequences of 5.1.3.6

First molecular characterization of a uridine diphosphate galacturonate 4-epimerase: an enzyme required for capsular biosynthesis in Streptococcus pneumoniae type 1

Munoz, R.; Lopez, R.; De Frutos, M.; Garcia, E.; Mol. Microbiol. 31, 703-713 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli
Streptococcus pneumoniae
Inhibitors
Inhibitors
Commentary
Organism
Structure
CDP-glucose
-
Streptococcus pneumoniae
galactose
-
Streptococcus pneumoniae
GDP-glucose
-
Streptococcus pneumoniae
GMP
-
Streptococcus pneumoniae
NaCl
progressive decrease in activity above 100 mM NaCl, activity drops below 50% at 250 mM
Streptococcus pneumoniae
p-hydroxymercuribenzoate
reactivation by NAD+ and 2-mercaptoethanol
Streptococcus pneumoniae
UDP
-
Streptococcus pneumoniae
UDP-galactose
-
Streptococcus pneumoniae
UDP-glucose
-
Streptococcus pneumoniae
UDP-mannose
-
Streptococcus pneumoniae
UDP-xylose
-
Streptococcus pneumoniae
UMP
-
Streptococcus pneumoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
UDP-D-glucuronate
pH 7.5, 37°C
Streptococcus pneumoniae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Streptococcus pneumoniae
80000
-
gel filtration
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Streptococcus pneumoniae
the enzyme is required for capsular biosynthesis
?
-
Streptococcus pneumoniae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus pneumoniae
-
type 1
-
Purification (Commentary)
Commentary
Organism
-
Streptococcus pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is required for capsular biosynthesis
653259
Streptococcus pneumoniae
?
-
653259
Streptococcus pneumoniae
?
UDP-D-glucuronate
equilibrium constant in direction of UDP-galacturonate formation is 1.3
653259
Streptococcus pneumoniae
UDP-D-galacturonate
-
653259
Streptococcus pneumoniae
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Streptococcus pneumoniae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
42
-
Streptococcus pneumoniae
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
60 min, about 35% loss of activity
Streptococcus pneumoniae
30
-
60 min, about 50% loss of activity
Streptococcus pneumoniae
37
-
60 min, about 75% loss of activity
Streptococcus pneumoniae
42
-
60 min, about 20% loss of activity
Streptococcus pneumoniae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptococcus pneumoniae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
10
pH 6.5: about 50% of maximal activity, pH 10.0: about 25% of maximal activity
Streptococcus pneumoniae
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
50 nM to 5 mM, does not affect activity
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Streptococcus pneumoniae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
50 nM to 5 mM, does not affect activity
Streptococcus pneumoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CDP-glucose
-
Streptococcus pneumoniae
galactose
-
Streptococcus pneumoniae
GDP-glucose
-
Streptococcus pneumoniae
GMP
-
Streptococcus pneumoniae
NaCl
progressive decrease in activity above 100 mM NaCl, activity drops below 50% at 250 mM
Streptococcus pneumoniae
p-hydroxymercuribenzoate
reactivation by NAD+ and 2-mercaptoethanol
Streptococcus pneumoniae
UDP
-
Streptococcus pneumoniae
UDP-galactose
-
Streptococcus pneumoniae
UDP-glucose
-
Streptococcus pneumoniae
UDP-mannose
-
Streptococcus pneumoniae
UDP-xylose
-
Streptococcus pneumoniae
UMP
-
Streptococcus pneumoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
UDP-D-glucuronate
pH 7.5, 37°C
Streptococcus pneumoniae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Streptococcus pneumoniae
80000
-
gel filtration
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Streptococcus pneumoniae
the enzyme is required for capsular biosynthesis
?
-
Streptococcus pneumoniae
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is required for capsular biosynthesis
653259
Streptococcus pneumoniae
?
-
653259
Streptococcus pneumoniae
?
UDP-D-glucuronate
equilibrium constant in direction of UDP-galacturonate formation is 1.3
653259
Streptococcus pneumoniae
UDP-D-galacturonate
-
653259
Streptococcus pneumoniae
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Streptococcus pneumoniae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
42
-
Streptococcus pneumoniae
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
60 min, about 35% loss of activity
Streptococcus pneumoniae
30
-
60 min, about 50% loss of activity
Streptococcus pneumoniae
37
-
60 min, about 75% loss of activity
Streptococcus pneumoniae
42
-
60 min, about 20% loss of activity
Streptococcus pneumoniae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptococcus pneumoniae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
10
pH 6.5: about 50% of maximal activity, pH 10.0: about 25% of maximal activity
Streptococcus pneumoniae
Other publictions for EC 5.1.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716581
Wojtasik
The changes in pectin metaboli ...
Linum usitatissimum
Plant Physiol. Biochem.
49
862-872
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
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-
1
-
-
1
-
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-
1
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-
1
-
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-
-
-
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-
-
-
1
1
1
1
-
-
716216
Pang
Comparative proteomics indicat ...
Arabidopsis thaliana, Gossypium hirsutum
Mol. Cell. Proteomics
9
2019-2033
2010
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
-
3
-
-
2
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2
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-
2
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
700488
Siddique
Myo-inositol oxygenase genes a ...
Arabidopsis thaliana
New Phytol.
184
457-472
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
-
-
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-
-
-
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-
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6
-
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-
-
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-
-
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-
12
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
704357
Gu
Enzymatic characterization and ...
Arabidopsis thaliana, Oryza sativa, Zea mays
J. Biochem.
146
527-534
2009
-
-
3
-
1
-
6
4
-
-
7
5
-
12
-
-
3
-
-
-
-
1
5
3
3
-
3
4
3
1
-
-
10
-
-
-
-
5
-
-
1
-
-
10
10
4
-
-
8
5
-
-
-
5
-
-
-
1
5
4
5
-
3
4
4
1
-
-
-
-
-
-
-
-
661739
Usadel
Identification and characteriz ...
Arabidopsis thaliana
FEBS Lett.
569
327-331
2004
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
8
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
663125
Molhoj
The biosynthesis of D-galactur ...
Arabidopsis thaliana
Plant Physiol.
135
1221-1230
2004
-
-
1
-
-
1
2
1
1
-
-
-
-
4
-
-
-
-
-
6
-
1
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
11
-
1
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
663134
Gu
The biosynthesis of UDP-galact ...
Arabidopsis thaliana
Plant Physiol.
136
4256-4264
2004
-
-
1
-
-
1
5
1
-
-
2
-
-
3
-
-
1
-
-
4
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
5
-
1
-
-
2
-
-
-
-
1
-
4
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
653259
Munoz
First molecular characterizati ...
Streptococcus pneumoniae
Mol. Microbiol.
31
703-713
1999
-
-
1
-
-
-
12
1
1
-
2
1
-
3
-
-
1
-
-
-
-
-
2
1
1
-
4
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
12
-
1
1
-
2
1
-
-
-
1
-
-
-
-
2
1
1
-
4
-
1
1
-
-
-
-
-
-
-
-
2403
Bolwell
-
Decrease of polygalacturonic a ...
Acer pseudoplatanus
Phytochemistry
24
699-702
1985
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
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-
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-
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-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2401
Dalessandro
Changes in enzymic activities ...
Abies grandis, Pinus sylvestris
Biochem. J.
162
281-288
1977
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
4
-
-
4
-
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-
2
-
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-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2405
Maitra
The mechanism of uridine dipho ...
Dolichospermum flos-aquae
J. Biol. Chem.
249
3075-3078
1974
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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1
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1
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-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
2406
Gaunt
Uridine diphosphate galacturon ...
Dolichospermum flos-aquae
J. Biol. Chem.
249
2366-2372
1974
-
-
-
-
-
-
14
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
1
-
-
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-
-
1
1
-
-
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-
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14
-
1
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-
1
-
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1
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-
1
-
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-
-
-
1
1
-
-
-
-
-
-
-
-
2407
Davidson
-
UDP-D-galacturonic acid 4-epim ...
Raphanus sativus
Methods Enzymol.
8
276-277
1966
-
-
-
-
-
-
-
-
-
-
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-
1
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1
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1
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1
1
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1
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1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2408
Feingold
The 4-epimerization and decarb ...
Vigna radiata var. radiata
J. Biol. Chem.
235
910-913
1960
-
-
-
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1
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1
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1
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1
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1
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