Cloned (Comment) | Organism |
---|---|
gene UXE1, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive | Albuca bracteata |
gene UXE2, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive | Albuca bracteata |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of truncated enzyme for expression as soluble protein. The truncated recombinant enzyme is catalytically inactive | Albuca bracteata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a type I membrane protein | Albuca bracteata | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-L-arabinose | Albuca bracteata | - |
UDP-D-xylose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Albuca bracteata | A0A168GPQ3 | UXE1; i.e. Albuca bracteata or Ornithogalum longebracteatum | - |
Albuca bracteata | A0A168GPR3 | UXE2; i.e. Albuca bracteata or Ornithogalum longebracteatum | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged truncated inactive enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Albuca bracteata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bulb | sterile | Albuca bracteata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme UXE, has strict substrate specificity and only catalyzes the interconversion of UDP-D-Xyl and UDP-L-Ara | Albuca bracteata | ? | - |
? | |
UDP-L-arabinose | - |
Albuca bracteata | UDP-D-xylose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 45400, about, sequence calculation | Albuca bracteata |
? | x * 46200, about, sequence calculation | Albuca bracteata |
Synonyms | Comment | Organism |
---|---|---|
UDP-xylose 4-epimerase | - |
Albuca bracteata |
Uxe | - |
Albuca bracteata |
UXE1 | - |
Albuca bracteata |
UXE2 | - |
Albuca bracteata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Albuca bracteata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Albuca bracteata |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Albuca bracteata | sequence calculation | - |
6.93 |
Albuca bracteata | sequence calculation | - |
9.05 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of anti-tumor polysaccharides in Ornithogalum caudatum. Enzyme UXE also plays a key role in carbohydrate partitioning, plant growth and development. UXE is important in the composition of the sugar nucleotide pools | Albuca bracteata |