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Literature summary for 5.1.3.5 extracted from
- Biosynthesis of UDP-xylose and UDP-arabinose in Sinorhizobium meliloti 1021: first characterization of a bacterial UDP-xylose synthase, and UDP-xylose 4-epimerase (2011), Microbiology, 157, 260-269.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Sinorhizobium meliloti |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is fully active in the presence of EDTA and is not inhibited by NADH | Sinorhizobium meliloti | |
| UDP-galactose | - |
Sinorhizobium meliloti |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
UDP-alpha-D-xylopyranose | apparent value, at 37°C, in 50 mM sodium phosphate, pH 7.6 | Sinorhizobium meliloti | |
| 0.31 | - |
UDP-D-xylopyranose | apparent value, at 37°C, in 50 mM sodium phosphate, pH 7.6 | Sinorhizobium meliloti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metal ions are not required for enzyme activity | Sinorhizobium meliloti |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36700 | - |
2 * 36700, calculated from amino acid sequence | Sinorhizobium meliloti |
| 66000 | - |
gel filtration | Sinorhizobium meliloti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | Q92WA3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | UDP-galactose, UDP-glucose, UDP-glucuronic acid, UDP-galacturonic acid, UDP-N-acetylglucosamine, and GDP-mannose are no substrates for the enzyme | Sinorhizobium meliloti | ? | - |
? | |
| UDP-alpha-D-xylopyranose | - |
Sinorhizobium meliloti | UDP-beta-L-arabinopyranose | - |
r | |
| UDP-D-xylopyranose | - |
Sinorhizobium meliloti | UDP-beta-L-arabinopyranose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 36700, calculated from amino acid sequence | Sinorhizobium meliloti |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-xylose 4-epimerase | - |
Sinorhizobium meliloti |
| Uxe | - |
Sinorhizobium meliloti |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Sinorhizobium meliloti |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13 | - |
UDP-alpha-D-xylopyranose | apparent value, at 37°C, in 50 mM sodium phosphate, pH 7.6 | Sinorhizobium meliloti | |
| 13 | - |
UDP-D-xylopyranose | apparent value, at 37°C, in 50 mM sodium phosphate, pH 7.6 | Sinorhizobium meliloti | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
in phosphate buffer | Sinorhizobium meliloti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Sinorhizobium meliloti | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 42.1 | - |
UDP-alpha-D-xylopyranose | apparent value, at 37°C, in 50 mM sodium phosphate, pH 7.6 | Sinorhizobium meliloti | |
| 42.1 | - |
UDP-D-xylopyranose | apparent value, at 37°C, in 50 mM sodium phosphate, pH 7.6 | Sinorhizobium meliloti | |
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