Literature summary for 5.1.3.37 extracted from

Wolfram, F.; Kitova, E.N.; Robinson, H.; Walvoort, M.T.; Codee, J.D.; Klassen, J.S.; Howell, P.L.
Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG (2014), J. Biol. Chem., 289, 6006-6019.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas syringae pv. tomato

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.1 A resolution. Isoform AlgG is a long right-handed parallel beta-helix with an elaborate lid structure. Residue His319 acts as the catalytic base and that Arg345 neutralizes the acidic group during the epimerase reaction. Water is the likely catalytic acid. Substrate docking studies suggest that a conserved electropositive groove facilitates polymannuronate binding and contains at least nine substrate binding subsite	Pseudomonas syringae pv. tomato

Crystallization (Comment)

Organism

to 2.1 A resolution. Isoform AlgG is a long right-handed parallel beta-helix with an elaborate lid structure. Residue His319 acts as the catalytic base and that Arg345 neutralizes the acidic group during the epimerase reaction. Water is the likely catalytic acid. Substrate docking studies suggest that a conserved electropositive groove facilitates polymannuronate binding and contains at least nine substrate binding subsite

Pseudomonas syringae pv. tomato

Protein Variants

Protein Variants	Comment	Organism
D317A	about 5% of wild-type activity	Pseudomonas syringae pv. tomato
D320A	complete loss of activity	Pseudomonas syringae pv. tomato
D368N	about 5% of wild-type activity	Pseudomonas syringae pv. tomato
D452A	about 70% of wild-type activity	Pseudomonas syringae pv. tomato
H319A	complete loss of activity	Pseudomonas syringae pv. tomato
H339A	about 50% of wild-type activity	Pseudomonas syringae pv. tomato
K338A	about 90% of wild-type activity	Pseudomonas syringae pv. tomato
R321K	about 75% of wild-type activity	Pseudomonas syringae pv. tomato
R345A	about 10% of wild-type activity	Pseudomonas syringae pv. tomato
R345K	about 40% of wild-type activity	Pseudomonas syringae pv. tomato
R345Q	about125% of wild-type activity	Pseudomonas syringae pv. tomato
R353E	complete loss of activity	Pseudomonas syringae pv. tomato
R369A	about 25% of wild-type activity	Pseudomonas syringae pv. tomato
R415C	complete loss of activity	Pseudomonas syringae pv. tomato
S344A	about 55% of wild-type activity	Pseudomonas syringae pv. tomato
Y291F	about 85% of wild-type activity	Pseudomonas syringae pv. tomato
Y294A	about 25% of wild-type activity	Pseudomonas syringae pv. tomato
Y294F	about 65% of wild-type activity	Pseudomonas syringae pv. tomato
Y296A	about 65% of wild-type activity	Pseudomonas syringae pv. tomato
Y314F	about 5% of wild-type activity	Pseudomonas syringae pv. tomato
Y392A	about 65% of wild-type activity	Pseudomonas syringae pv. tomato
Y392F	about 70% of wild-type activity	Pseudomonas syringae pv. tomato

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Pseudomonas syringae pv. tomato	-	-

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas syringae pv. tomato	Q887Q3	-	-
Pseudomonas syringae pv. tomato DC3000	Q887Q3	-	-