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Literature summary for 5.1.3.31 extracted from
- Characterization of D-tagatose-3-epimerase from Rhodobacter sphaeroides that converts D-fructose into D-psicose (2009), Biotechnol. Lett., 31, 857-862.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Cereibacter sphaeroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Cereibacter sphaeroides |  |
| Zn2+ | - |
Cereibacter sphaeroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required, strongly enhances activity | Cereibacter sphaeroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Cereibacter sphaeroides |
| 64000 | - |
gel filtration | Cereibacter sphaeroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | C1KKR1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | specificity is highest with D-fructose and decreases for other substrates in the order: D-tagatose, D-psicose, D-ribulose, D-xylulose and D-sorbose. The equilibrium ratio between D-psicose and D-fructose is 23:77 after 24 h at 40°C | Cereibacter sphaeroides | D-psicose | - |
r | |
| D-ribulose | specificity is highest with D-fructose and decreases for other substrates in the order: D-tagatose, D-psicose, D-ribulose, D-xylulose and D-sorbose | Cereibacter sphaeroides | D-xylulose | - |
? | |
| D-tagatose | specificity is highest with D-fructose and decreases for other substrates in the order: D-tagatose, D-psicose, D-ribulose, D-xylulose and D-sorbose | Cereibacter sphaeroides | D-sorbose | - |
r | |
| additional information | no activity with D-fructose 6-phosphate and D-ribulose 5-phosphate | Cereibacter sphaeroides | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Cereibacter sphaeroides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 70% of maximal activity, 50°C: about 70% of maximal activity | Cereibacter sphaeroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
4 h, less than 10% loss of activity | Cereibacter sphaeroides |
| 45 | - |
4 h, about 65% loss of activity | Cereibacter sphaeroides |
| 50 | - |
1 h, about 50% loss of activity | Cereibacter sphaeroides |
| 55 | - |
30 min, about 80% loss of activity | Cereibacter sphaeroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Cereibacter sphaeroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | pH 8.0: about 75% of maximal activity, pH 10.0: about 70% of maximal activity | Cereibacter sphaeroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
4°C, 1 h, 65% loss of activity | Cereibacter sphaeroides |
| 7 | - |
4°C, 1 h, 25% loss of activity | Cereibacter sphaeroides |
| 8 | 10 | 4°C, 1 h, stable | Cereibacter sphaeroides |
| 11 | - |
4°C, 1 h, 30% loss of activity | Cereibacter sphaeroides |
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Release 2023.1 (January 2023)
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