Literature summary for 5.1.3.30 extracted from

Jia, M.; Mu, W.; Chu, F.; Zhang, X.; Jiang, B.; Zhou, L.; Zhang, T.
A D-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for D-psicose production Cloning, expression, purification, and characterization (2014), Appl. Microbiol. Biotechnol., 98, 717-725 .

Search for more literature for 5.1.3.30

Application

Application	Comment	Organism
synthesis	the enzyme is a potential D-psicose producer for industrial production	Enterocloster bolteae

Cloned(Commentary)

Cloned (Comment)	Organism
gene CLOBOL_00069, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli	Enterocloster bolteae

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme overexpression for heterologous production of D-psicose from D-fructose, under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31. For high production of D-psicose, 216 g/L D-psicose are produced with 28.8% turnover yield at pH 6.5 and 55°C. The recombinant DPEase exhibits weak acid stability and thermostability and has a high affinity and turnover for the substrate D-fructose	Enterocloster bolteae

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Enterocloster bolteae
EDTA	complete inhibition	Enterocloster bolteae
Mg2+	-	Enterocloster bolteae
Zn2+	-	Enterocloster bolteae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
27.4	-	D-psicose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae
59.8	-	D-fructose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae
339	-	D-tagatose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activates most highly at 0.5 mM, 80% of maximal activation at 1-5 mM, best divalent cation, dependent on	Enterocloster bolteae
Mn2+	activates	Enterocloster bolteae
additional information	the CB-DPEase is a metalloprotein, that is inactive in absence of divalent metal ions	Enterocloster bolteae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-psicose	Enterocloster bolteae	-	D-fructose	-	r
D-psicose	Enterocloster bolteae ATCC BAA-613	-	D-fructose	-	r

Organism

Organism	UniProt	Comment	Textmining
Enterocloster bolteae	A8RG82	-	-
Enterocloster bolteae ATCC BAA-613	A8RG82	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity	Enterocloster bolteae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose	66.4% activity compared to the activity with D-psicose	Enterocloster bolteae	D-psicose	-	r
D-fructose	66.4% activity compared to the activity with D-psicose	Enterocloster bolteae ATCC BAA-613	D-psicose	-	r
D-psicose	-	Enterocloster bolteae	D-fructose	-	r
D-psicose	i.e. D-ribo-2-hexulose or D-allulose, best substrate	Enterocloster bolteae	D-fructose	-	r
D-psicose	-	Enterocloster bolteae ATCC BAA-613	D-fructose	-	r
D-psicose	i.e. D-ribo-2-hexulose or D-allulose, best substrate	Enterocloster bolteae ATCC BAA-613	D-fructose	-	r
D-sorbose	1.2% activity compared to the activity with D-psicose	Enterocloster bolteae	D-tagatose	-	r
D-sorbose	1.2% activity compared to the activity with D-psicose	Enterocloster bolteae ATCC BAA-613	D-tagatose	-	r
D-tagatose	23.2% activity compared to the activity with D-psicose	Enterocloster bolteae	D-sorbose	-	r
D-tagatose	23.2% activity compared to the activity with D-psicose	Enterocloster bolteae ATCC BAA-613	D-sorbose	-	r

Subunits

Subunits	Comment	Organism
?	x * 33015, sequence calculation	Enterocloster bolteae

Synonyms

Synonyms	Comment	Organism
CB-DPEase	-	Enterocloster bolteae
CLOBOL_00069	-	Enterocloster bolteae
DPEase	-	Enterocloster bolteae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	recombinant enzyme	Enterocloster bolteae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	purified recombinant enzyme, half-life is 156 min	Enterocloster bolteae
100	-	purified recombinant enzyme, 10 min, inactivation	Enterocloster bolteae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.7	-	D-tagatose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae
49	-	D-psicose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae
59	-	D-fructose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	recombinant enzyme	Enterocloster bolteae

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	9	recombinant enzyme, activity range, profile overview	Enterocloster bolteae

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	9	purified recombinant enzyme, 80-100% of maximal activity within this range	Enterocloster bolteae
7	-	purified recombinant enzyme, most stable at	Enterocloster bolteae

pI Value

Organism	Comment	pI Value Maximum	pI Value
Enterocloster bolteae	sequence calculation	-	5.02

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.014	-	D-tagatose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae
0.99	-	D-fructose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae
1.78	-	D-psicose	pH 7.0, 55°C, recombinant enzyme	Enterocloster bolteae

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Release 2023.1 (January 2023)