Application | Comment | Organism |
---|---|---|
synthesis | useful in the bioproduction of D-psicose, a rare hexose sugar, from D-fructose, found plenty in nature | Ruminiclostridium cellulolyticum |
Crystallization (Comment) | Organism |
---|---|
sitting drop method. The crystal structure of the apoenzyme and the enzyme in complex with substrates or products (D-psicose, D-fructose, D-tagatose and D-sorbose). From the complex structures of the enzyme with D-psicose and D-fructose, the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues. Accordingly, based on these ketohexosebound complex structures, a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed. These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis | Ruminiclostridium cellulolyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminiclostridium cellulolyticum | B8I944 | - |
- |
Ruminiclostridium cellulolyticum ATCC 35319 | B8I944 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Ruminiclostridium cellulolyticum | D-psicose | - |
r | |
D-fructose | - |
Ruminiclostridium cellulolyticum ATCC 35319 | D-psicose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
DPEase | ambiguous | Ruminiclostridium cellulolyticum |