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Literature summary for 5.1.3.30 extracted from

  • Chan, H.C.; Zhu, Y.; Hu, Y.; Ko, T.P.; Huang, C.H.; Ren, F.; Chen, C.C.; Ma, Y.; Guo, R.T.; Sun, Y.
    Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars (2012), Protein Cell, 3, 123-131.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis useful in the bioproduction of D-psicose, a rare hexose sugar, from D-fructose, found plenty in nature Ruminiclostridium cellulolyticum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method. The crystal structure of the apoenzyme and the enzyme in complex with substrates or products (D-psicose, D-fructose, D-tagatose and D-sorbose). From the complex structures of the enzyme with D-psicose and D-fructose, the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues. Accordingly, based on these ketohexosebound complex structures, a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed. These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis Ruminiclostridium cellulolyticum

Organism

Organism UniProt Comment Textmining
Ruminiclostridium cellulolyticum B8I944
-
-
Ruminiclostridium cellulolyticum ATCC 35319 B8I944
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose
-
Ruminiclostridium cellulolyticum D-psicose
-
r
D-fructose
-
Ruminiclostridium cellulolyticum ATCC 35319 D-psicose
-
r

Synonyms

Synonyms Comment Organism
DPEase ambiguous Ruminiclostridium cellulolyticum