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Literature summary for 5.1.3.30 extracted from

  • Mu, W.; Zhang, W.; Fang, D.; Zhou, L.; Jiang, B.; Zhang, T.
    Characterization of a D-psicose-producing enzyme, D-psicose 3-epimerase, from Clostridium sp. (2013), Biotechnol. Lett., 35, 1481-1486.
    View publication on PubMed
Search for more literature for 5.1.3.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Clostridium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
227.6
-
 D-psicose pH 8.0, 65°C, in presence of 0.2 mM Co2+ Clostridium sp.
279
-
 D-fructose pH 8.0, 65°C, in presence of 0.2 mM Co2+ Clostridium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the metal-dependent enzyme requires Co2+ as optimum cofactor. The relative activity is linearly increased when adding Co2+ from 0.005 mM to 0.035 mM. When adding greater concentrations of Co2+, the relative activity tends to be stable. The optimum Co2+ concentration is 0.2 mM for catalytic activity Clostridium sp.
Mg2+ the metal-dependent enzyme requires Co2+ as optimum cofactor. When Co2+ is replaced with Mg2+ the enzyme activity is reduced to 22% of that in presence of Co2+ Clostridium sp.
Mn2+ the metal-dependent enzyme requires Co2+ as optimum cofactor. When Co2+ is replaced with Mn2+ the enzyme activity is reduced to 66% of that in presence of Co2+ Clostridium sp.
Ni2+ the metal-dependent enzyme requires Co2+ as optimum cofactor. When Co2+ is replaced with Ni2+ the enzyme activity is reduced to 31% of that in presence of Co2+ Clostridium sp.

Organism

Organism UniProt Comment Textmining
Clostridium sp. H2JEN2
-
-
Clostridium sp. BNL1100 H2JEN2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity chromatography Clostridium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-psicose optimum substrate. Equilibrium ratio between D-psicose and D-fructose is 28:72 at 65°C Clostridium sp. D-fructose
-
 r
D-psicose optimum substrate. Equilibrium ratio between D-psicose and D-fructose is 28:72 at 65°C Clostridium sp. BNL1100 D-fructose
-
 r

Synonyms

Synonyms Comment Organism
Clo1100_1157 locus name Clostridium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Clostridium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 75 50°C: about 50% of maximal activity, 75°C: about 70% of maximal activity Clostridium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 4 h, stable Clostridium sp.
50
-
 4 h, about 50% loss of activity Clostridium sp.
60
-
 half-life without metal: 15 min Clostridium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
273
-
 D-fructose pH 8.0, 65°C, in presence of 0.2 mM Co2+ Clostridium sp.
536
-
 D-psicose pH 8.0, 65°C, in presence of 0.2 mM Co2+ Clostridium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Clostridium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 80% of maximal activity, pH 9.0: about 90% of maximal activity Clostridium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 4°C, 2 h, more than 80% of initial activity remains Clostridium sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.98
-
 D-fructose pH 8.0, 65°C, in presence of 0.2 mM Co2+ Clostridium sp.
2.4
-
 D-psicose pH 8.0, 65°C, in presence of 0.2 mM Co2+ Clostridium sp.