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Literature summary for 5.1.3.3 extracted from

  • You, W.; Qiu, X.; Zhang, Y.; Ma, J.; Gao, Y.; Zhang, X.; Niu, L.; Teng, M.
    Crystallization and preliminary X-ray diffraction analysis of the putative aldose 1-epimerase YeaD from Escherichia coli (2010), Acta Crystallogr. Sect. F, 66, 951-953.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 200 mM sodium acetate and 100 mM Tris-HCl pH 8.5 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography and Superdex 200 gel filtration Escherichia coli

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
YeaD
-
Escherichia coli