Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 200 mM sodium acetate and 100 mM Tris-HCl pH 8.5 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography and Superdex 200 gel filtration | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
YeaD | - |
Escherichia coli |