Cloned (Comment) | Organism |
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efficient stereospecific enzymatic synthesis of L-[15N]-valine, L-[15N]-leucine, L-[15N]-norvaline, L-[15N]-norleucine and L-[15N]-isoleucine from the corresponding alpha-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale of 1-10 g. Substrates alpha-ketoisovalerate, alpha-ketoisocaproate, alpha-ketovalerate, alpha-ketocaproateand alpha-keto-beta-methylvalerate are converted to products L-valine, L-leucine, L-norvaline, L-isoleucine with yields of 90%, 92.5%, 92%, 80%, and 95%, respectively | Escherichia coli |
Organism | UniProt | Comment | Textmining |
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Escherichia coli | - |
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- |
Synonyms | Comment | Organism |
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GalM | - |
Escherichia coli |