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Literature summary for 5.1.3.3 extracted from

  • Thoden, J.B.; Kim, J.; Raushel, F.M.; Holden, H.M.
    Structural and kinetic studies of sugar binding to galactose mutarotase from Lactococcus lactis (2002), J. Biol. Chem., 277, 45458-45465.
    View publication on PubMed
Search for more literature for 5.1.3.3

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with D-glucose, D-fucose, D-quinovose, L-arabinose, or D-xylose Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
20
-
 alpha-D-galactose pH 7.6, 25°C Lactococcus lactis
21
-
 alpha-D-xylose pH 7.6, 25°C Lactococcus lactis
25
-
 alpha-D-Fucose pH 7.6, 25°C Lactococcus lactis
32
-
 alpha-L-arabinose pH 7.6, 25°C Lactococcus lactis
34
-
 alpha-D-glucose pH 7.6, 25°C Lactococcus lactis
37
-
 alpha-D-quinovose pH 7.6, 25°C Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 recombinant protein expressed in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-fucose
-
 Lactococcus lactis beta-D-fucose
-
 ?
alpha-D-galactose
-
 Lactococcus lactis beta-D-galactose
-
 ?
alpha-D-glucose
-
 Lactococcus lactis beta-D-glucose
-
 ?
alpha-D-quinovose
-
 Lactococcus lactis beta-D-quinovose
-
 ?
alpha-D-xylose
-
 Lactococcus lactis beta-D-xylose
-
 ?
alpha-L-arabinose
-
 Lactococcus lactis beta-L-arabinose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
410
-
 alpha-D-xylose pH 7.6, 25°C Lactococcus lactis
430
-
 alpha-D-glucose pH 7.6, 25°C Lactococcus lactis
490
-
 alpha-D-quinovose pH 7.6, 25°C Lactococcus lactis
1700
-
 alpha-D-Fucose pH 7.6, 25°C Lactococcus lactis
3700
-
 alpha-D-galactose pH 7.6, 25°C Lactococcus lactis
5500
-
 alpha-L-arabinose pH 7.6, 25°C Lactococcus lactis