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Literature summary for 5.1.3.3 extracted from

  • Timson, D.J.; Reece, R.J.
    Identification and characterisation of human aldose 1-epimerase (2003), FEBS Lett., 543, 21-24.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E307A mutant enzyme without aldose 1-epimerase activity Homo sapiens
H107A mutant enzyme with 5fold reduced activity with alpha-D-galactose and 7.8fold reduced activity with alpha-D-glucose, compared to wild-type enzyme Homo sapiens
H176A mutant enzyme without aldose 1-epimerase activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
37
-
alpha-D-galactose pH 8.0, 20°C Homo sapiens
54
-
alpha-D-glucose pH 8.0, 20°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens key enzyme of carbohydrate metabolism catalysing the interconversion of the alpha-anomer and beta-anomer of hexose sugars such as glucose and galactose ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96C23
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galactose
-
Homo sapiens beta-D-galactose
-
r
alpha-D-glucose
-
Homo sapiens beta-D-glucose
-
r
additional information key enzyme of carbohydrate metabolism catalysing the interconversion of the alpha-anomer and beta-anomer of hexose sugars such as glucose and galactose Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4900
-
alpha-D-glucose pH 8.0, 20°C Homo sapiens
12000
-
alpha-D-galactose pH 8.0, 20°C Homo sapiens