Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E307A | mutant enzyme without aldose 1-epimerase activity | Homo sapiens |
H107A | mutant enzyme with 5fold reduced activity with alpha-D-galactose and 7.8fold reduced activity with alpha-D-glucose, compared to wild-type enzyme | Homo sapiens |
H176A | mutant enzyme without aldose 1-epimerase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37 | - |
alpha-D-galactose | pH 8.0, 20°C | Homo sapiens | |
54 | - |
alpha-D-glucose | pH 8.0, 20°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | key enzyme of carbohydrate metabolism catalysing the interconversion of the alpha-anomer and beta-anomer of hexose sugars such as glucose and galactose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96C23 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-galactose | - |
Homo sapiens | beta-D-galactose | - |
r | |
alpha-D-glucose | - |
Homo sapiens | beta-D-glucose | - |
r | |
additional information | key enzyme of carbohydrate metabolism catalysing the interconversion of the alpha-anomer and beta-anomer of hexose sugars such as glucose and galactose | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4900 | - |
alpha-D-glucose | pH 8.0, 20°C | Homo sapiens | |
12000 | - |
alpha-D-galactose | pH 8.0, 20°C | Homo sapiens |