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Literature summary for 5.1.3.3 extracted from
- Purification and properties of aldose 1-epimerase from Aspergillus niger (1981), Biochim. Biophys. Acta, 662, 285-290.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | rapid determination of glucose by aldose 1-epimerase | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
alpha-D-glucose | - |
Aspergillus niger |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
2 * 130000, SDS-PAGE | Aspergillus niger |
| 260000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-galactose | 105% of the activity relative to alpha-D-glucose | Aspergillus niger | beta-D-galactose | - |
? | |
| alpha-D-glucose | - |
Aspergillus niger | beta-D-glucose | - |
? | |
| alpha-D-xylose | 31% of the activity relative to alpha-D-glucose | Aspergillus niger | beta-D-xylose | - |
? | |
| alpha-L-arabinose | beta-L-arabinose is epimerized at 108% of the activity relative to alpha-D-glucose | Aspergillus niger | beta-L-arabinose | - |
? | |
| beta-D-Cellobiose | 15% of the activity relative to alpha-D-glucose | Aspergillus niger | ? | - |
? | |
| beta-D-Fructose | 135% of the activity relative to alpha-D-glucose | Aspergillus niger | alpha-D-Fructose | - |
? | |
| beta-Lactose | 10% of the activity relative to alpha-D-glucose | Aspergillus niger | alpha-Lactose | - |
? | |
| beta-Maltose | 13% of the activity relative to alpha-D-glucose | Aspergillus niger | alpha-Maltose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 130000, SDS-PAGE | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
30 min, stable below | Aspergillus niger |
| 60 | - |
30 min, 5% loss of activity | Aspergillus niger |
| 65 | - |
30 min, 25% loss of activity | Aspergillus niger |
| 75 | - |
half-life: 6.9 min | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2600 | - |
alpha-D-glucose | - |
Aspergillus niger | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
20°C, 24 h, complete loss of activity | Aspergillus niger |
| 3 | - |
20°C, 24 h, 50% loss of activity | Aspergillus niger |
| 5.5 | 9 | 20°C, 24 h, no inactivation | Aspergillus niger |
| 10.5 | - |
20°C, 24 h, 66% loss of activity | Aspergillus niger |
| 11 | - |
20°C, 24 h, complete loss of activity | Aspergillus niger |
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