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Literature summary for 5.1.3.3 extracted from

  • Chang, H.N.; Reilly, P.J.
    Immobilization and characterization of hog kidney mutarotase (1977), Biotechnol. Bioeng., 19, 923-928.
    View publication on PubMed

General Stability

General Stability Organism
immobilization to porious silica does not stabilize the enzyme to a sufficient degree that it can be used for long periods of time Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
40
-
alpha-D-glucose immobilized enzyme Sus scrofa

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Sus scrofa
-
hog
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney cortex Sus scrofa
-
kidney cortex Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose
-
Sus scrofa beta-D-glucose
-
?
alpha-D-glucose
-
Bos taurus beta-D-glucose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55 20°C: about 50% of maximal activity, 55°C: about 80% of maximal activity, immobilized enzyme Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 55 immobilized hog kidney enzyme is 5 to 50 times more stable as the soluble form of the closely related bovine kidney enzyme Sus scrofa
50 55 immobilized hog kidney enzyme is 5 to 50 times more stable as the soluble form of the closely related bovine kidney enzyme Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8 free and immobilized enzyme Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 9 6.0: about 70% of maximal activity, free enzyme. About 90% of maximal activity, immobilized enzyme, 9: about 40% of maximal activity, free enzyme. About 60% of maximal activity, immobilized enzyme Sus scrofa