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Literature summary for 5.1.3.22 extracted from

  • Le, S.B.; Heggeset, T.M.B.; Haugen, T.; Naerdal, I.; Brautaset, T.
    6-Phosphofructokinase and ribulose-5-phosphate 3-epimerase in methylotrophic Bacillus methanolicus ribulose monophosphate cycle (2017), Appl. Microbiol. Biotechnol., 101, 4185-4200 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus methanolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Bacillus methanolicus
Mn2+ stimulates Bacillus methanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ribulose 5-phosphate Bacillus methanolicus the enzyme is involved in the ribulose monophosphate cycle L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate Bacillus methanolicus PB1 / NCIMB13113 the enzyme is involved in the ribulose monophosphate cycle L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate Bacillus methanolicus PB1 the enzyme is involved in the ribulose monophosphate cycle L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate Bacillus methanolicus MGA3 the enzyme is involved in the ribulose monophosphate cycle L-xylulose 5-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus
-
-
-
Bacillus methanolicus I3DTP3
-
-
Bacillus methanolicus MGA3
-
-
-
Bacillus methanolicus PB1 I3DTP3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ribulose 5-phosphate
-
Bacillus methanolicus L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate
-
Bacillus methanolicus PB1 / NCIMB13113 L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus PB1 / NCIMB13113 L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate
-
Bacillus methanolicus PB1 L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus PB1 L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate
-
Bacillus methanolicus MGA3 L-xylulose 5-phosphate
-
?
L-ribulose 5-phosphate the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus MGA3 L-xylulose 5-phosphate
-
?

Subunits

Subunits Comment Organism
hexamer
-
Bacillus methanolicus

Synonyms

Synonyms Comment Organism
ribulose-5-phosphate 3-epimerase
-
Bacillus methanolicus
RPE
-
Bacillus methanolicus
rpe1 wild-type strain MGA3, encoded on plasmid pBM19 Bacillus methanolicus
rpe1 wild-type strain PB1, encoded on plasmid pBM20 Bacillus methanolicus
rpe2 wild-type strain MGA3, chromosomal enzyme Bacillus methanolicus

Expression

Organism Comment Expression
Bacillus methanolicus transcription of the plasmid-encoded genes is 10fold to 15fold upregulated in cells growing on methanol compared to mannitol, while the chromosomal genes are transcribed at similar levels under both conditions up

General Information

General Information Comment Organism
physiological function the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus