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Literature summary for 5.1.3.20 extracted from

  • Wang, J.; Ma, W.; Wang, Z.; Li, Y.; Wang, X.
    Construction and characterization of an Escherichia coli mutant producing Kdo2-lipid A (2014), Mar. Drugs, 12, 1495-1511 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of the mutant Escherichia coli strains WBB06 and WJW00, that can synthesize Kdo2-lipid A, by deleting the rfaD gene from the genome of Escherichia coli wild-type strain W3110. 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo)2-lipid A is the conserved structure domain of lipopolysaccharide found in most Gram-negative bacteria, and is believed to stimulate the human innate immune system through the TLR4/MD2 complex. Kdo2-lipid A is an important stimulator for studying the mechanism of the innate immune system and for developing bacterial vaccine adjuvants. Compared with the wild-type strain W3110, WJW00 shows increased hydrophobicity, higher cell permeability, greater autoaggregation and decreased biofilm-forming ability Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-D-glycero-D-manno-heptose Escherichia coli
-
ADP-L-glycero-D-manno-heptose
-
r
ADP-D-glycero-D-manno-heptose Escherichia coli W3110 / K-12
-
ADP-L-glycero-D-manno-heptose
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P67910
-
-
Escherichia coli W3110 / K-12 P67910
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-D-glycero-D-manno-heptose
-
Escherichia coli ADP-L-glycero-D-manno-heptose
-
r
ADP-D-glycero-D-manno-heptose
-
Escherichia coli W3110 / K-12 ADP-L-glycero-D-manno-heptose
-
r

Synonyms

Synonyms Comment Organism
ADP-L-glycero-D-manno-heptose-6-epimerase
-
Escherichia coli
RfaD
-
Escherichia coli

General Information

General Information Comment Organism
malfunction the Escherichia coli rfaD-deletion mutant strain WJW00 can synthesize Kdo2-lipid A. 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo)2-lipid A is the conserved structure domain of lipopolysaccharide found in most Gram-negative bacteria, and is believed to stimulate the human innate immune system through the TLR4/MD2 complex. Kdo2-lipid A is an important stimulator for studying the mechanism of the innate immune system and for developing bacterial vaccine adjuvants. Compared with the wild-type strain W3110, WJW00 shows increased hydrophobicity, higher cell permeability, greater autoaggregation and decreased biofilm-forming ability. Phenotypes, overview Escherichia coli