Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the mutant Escherichia coli strains WBB06 and WJW00, that can synthesize Kdo2-lipid A, by deleting the rfaD gene from the genome of Escherichia coli wild-type strain W3110. 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo)2-lipid A is the conserved structure domain of lipopolysaccharide found in most Gram-negative bacteria, and is believed to stimulate the human innate immune system through the TLR4/MD2 complex. Kdo2-lipid A is an important stimulator for studying the mechanism of the innate immune system and for developing bacterial vaccine adjuvants. Compared with the wild-type strain W3110, WJW00 shows increased hydrophobicity, higher cell permeability, greater autoaggregation and decreased biofilm-forming ability | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-D-glycero-D-manno-heptose | Escherichia coli | - |
ADP-L-glycero-D-manno-heptose | - |
r | |
ADP-D-glycero-D-manno-heptose | Escherichia coli W3110 / K-12 | - |
ADP-L-glycero-D-manno-heptose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P67910 | - |
- |
Escherichia coli W3110 / K-12 | P67910 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-D-glycero-D-manno-heptose | - |
Escherichia coli | ADP-L-glycero-D-manno-heptose | - |
r | |
ADP-D-glycero-D-manno-heptose | - |
Escherichia coli W3110 / K-12 | ADP-L-glycero-D-manno-heptose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ADP-L-glycero-D-manno-heptose-6-epimerase | - |
Escherichia coli |
RfaD | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | the Escherichia coli rfaD-deletion mutant strain WJW00 can synthesize Kdo2-lipid A. 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo)2-lipid A is the conserved structure domain of lipopolysaccharide found in most Gram-negative bacteria, and is believed to stimulate the human innate immune system through the TLR4/MD2 complex. Kdo2-lipid A is an important stimulator for studying the mechanism of the innate immune system and for developing bacterial vaccine adjuvants. Compared with the wild-type strain W3110, WJW00 shows increased hydrophobicity, higher cell permeability, greater autoaggregation and decreased biofilm-forming ability. Phenotypes, overview | Escherichia coli |