Cloned (Comment) | Organism |
---|---|
gene rfaD, sequence compariosns, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Burkholderia thailandensis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 200 nl of 8.8 mg/ml protein in 0.43 M NaCl, 20 mM Tris-HCl, pH 8.0, with 200 nl of reservoir solution containing 35% PEG 200, 0.1 M bis-tris-HCl, pH 5.5, and equilibration against 0.05 ml of reservori solution, method optimization, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement | Burkholderia thailandensis |
Protein Variants | Comment | Organism |
---|---|---|
E210G | site-directed mutagenesis, structure compared to the wild-type enzyme | Burkholderia thailandensis |
K178M | site-directed mutagenesis, structure compared to the wild-type enzyme | Burkholderia thailandensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, recombinant His6-tagged enzyme, SDS-PAGE | Burkholderia thailandensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-D-glycero-D-manno-heptose | Burkholderia thailandensis | - |
ADP-L-glycero-D-manno-heptose | - |
r | |
ADP-D-glycero-D-manno-heptose | Burkholderia thailandensis E444 | - |
ADP-L-glycero-D-manno-heptose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia thailandensis | Q2SY18 | gene rfaD | - |
Burkholderia thailandensis E444 | Q2SY18 | gene rfaD | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Burkholderia thailandensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose | catalytic one-base reaction mechanism from docking study, overview. The key point in the catalytic process of AGME is the inversion at the C6'' stereocentre of ADP-D,D-Hep mediated by the reversible redox reactions of NADP+/NADPH | Burkholderia thailandensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-D-glycero-D-manno-heptose | - |
Burkholderia thailandensis | ADP-L-glycero-D-manno-heptose | - |
r | |
ADP-D-glycero-D-manno-heptose | - |
Burkholderia thailandensis E444 | ADP-L-glycero-D-manno-heptose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, recombinant His6-tagged enzyme, SDS-PAGE | Burkholderia thailandensis |
More | enzyme structure comparisons, detailed overview | Burkholderia thailandensis |
Synonyms | Comment | Organism |
---|---|---|
ADP-L-glycero-D-manno-heptose 6-epimerase | - |
Burkholderia thailandensis |
AGME | - |
Burkholderia thailandensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme contains a catalytic triad involved in catalyzing hydride transfer with the aid of NADP+ | Burkholderia thailandensis | |
NADPH | - |
Burkholderia thailandensis |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme contains a catalytic triad involved in catalyzing hydride transfer with the aid of NADP+. The enzyme may recognize their substrate in a lock-and-key fashion, binding analysis, docking study, enzyme structure comparisons, detailed overview | Burkholderia thailandensis |