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Literature summary for 5.1.3.2 extracted from

  • Shah, B.; Ashwood, H.; Harrop, S.; Farrugia, D.; Paulsen, I.; Mabbutt, B.
    Crystal structure of a UDP-GlcNAc epimerase for surface polysaccharide biosynthesis in Acinetobacter baumannii (2018), PLoS ONE, 13, e0191610 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
gene fnlA or ABD1_580, DNA and amino acid sequence determination and analysis, sequence comparisons, the gene belongs to the components of polysaccharide biosynthesis cluster RGP01, overview, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS Acinetobacter baumannii

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme Ab-WbjB, hanging drop vapour diffusion method, in an 1:1 ratio mixture from 0.2 M NaCl, 0.1 M HEPES, pH 5.6, and 25% v/v PEG 3350, X-ray diffraction structure determination and analysis at 2.64-2.70 A, method optimization, overview, molecular replacement using the structure of Helicobacter pylori FlaA1 (PDB ID 2gn4) as template Acinetobacter baumannii

Protein Variants

Protein Variants Comment Organism
M134A site-directed mutagenesis Acinetobacter baumannii
M134Y site-directed mutagenesis Acinetobacter baumannii

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii V5RBP5
-
-
Acinetobacter baumannii Dl279779 V5RBP5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography Acinetobacter baumannii

Subunits

Subunits Comment Organism
hexamer enzyme Ab-WbjB is a hexamer, organised into a trimer of chain pairs, with coenzyme NADP+ occupying each chain Acinetobacter baumannii

Synonyms

Synonyms Comment Organism
ABD1_580 locus name Acinetobacter baumannii
epimerase Ab-WbjB
-
 Acinetobacter baumannii
fnlA
-
 Acinetobacter baumannii
UDP-N-acetyl-glucosamine 4,6-dehydratase
-
 Acinetobacter baumannii
WbjB
-
 Acinetobacter baumannii

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Acinetobacter baumannii

General Information

General Information Comment Organism
evolution the epimerase and dehydratase Ab-WbjB belongs to the extended short-chain dehydrogenase/reductase (SDR) family, related in fold to previously characterised enzymes CapE and FlaA1 Acinetobacter baumannii