Cloned (Comment) | Organism |
---|---|
gene OsUGE-1, phylogenetic analysis of the UGEs from Oryza sativa, recombinant overexpression in Oryza sativa via Agrobacterium tumefaciens-mediated transformation, real-time PCR enzyme expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of transgenic rice plants to constitutively overexpress the OsUGE-1 gene (OsUGE1-OX1-2). The transgenic rice lines are similar in size to wild-type plants at the vegetative stage and at maturity regardless of the N-level tested. OsUGE1-OX lines maintain 18-24% more sucrose and 12-22% less cellulose in shoots compared to wild-type when subjected to suboptimal N-levels. OsUGE1-OX lines maintain proportionally more galactose and glucose in the hemicellulosic polysaccharide profile of plants compared to wild-type plants when grown under low N. The altered cell wall C-partitioning during N-limitation in the OsUGE1-OX lines appears to be mediated by OsUGE1 via the repression of the cellulose synthesis associated genes, OsSus1, OsCesA4, 7, and 9. Seed characteristics of rice plants overexpressing OsUGE1 compared to wild-type rice, overview | Oryza sativa Japonica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Oryza sativa Japonica Group | 5618 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose | Oryza sativa Japonica Group | - |
UDP-galactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q8LNZ3 | cv. Kaybonnet | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Oryza sativa Japonica Group |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme expression in relation to N levels in roots and shoots, overview | Oryza sativa Japonica Group | - |
root | - |
Oryza sativa Japonica Group | - |
seedling | - |
Oryza sativa Japonica Group | - |
shoot | - |
Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose | - |
Oryza sativa Japonica Group | UDP-galactose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
OsUGE-1 | - |
Oryza sativa Japonica Group |
OsUGE1 | - |
Oryza sativa Japonica Group |
UDP-glucose 4-epimerase 1 | - |
Oryza sativa Japonica Group |
UGE-1 | - |
Oryza sativa Japonica Group |
uridine-diphospho-glucose 4-epimerase | - |
Oryza sativa Japonica Group |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryza sativa Japonica Group |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Oryza sativa Japonica Group |
General Information | Comment | Organism |
---|---|---|
evolution | the rice genome contains four putative UGE-encoding genes (OsUGE1-4). All four predicted OsUGEs in rice carry an epimerase domain and belong to the NAD+ dependent epimerase/dehydratase family proteins that use NAD+ as a cofactor and nucleotide-sugars as substrates. Expression profiles of UGEs in Oryza sativa support distinct in planta roles throughout development | Oryza sativa Japonica Group |
malfunction | enzyme overexpressing mutant OsUGE1-OX lines maintain 18-24% more sucrose and 12-22% less cellulose in shoots compared to wild-type when subjected to suboptimal N-levels. OsUGE1-OX lines maintain proportionally more galactose and glucose in the hemicellulosic polysaccharide profile of plants compared to wild-type plants when grown under low N. The altered cell wall C-partitioning during N-limitation in the OsUGE1-OX lines appears to be mediated by OsUGE1 via the repression of the cellulose synthesis associated genes, OsSus1, OsCesA4, 7, and 9. OsUGE1 shows phenotypic alteration at carbohydrate partitioning level in the transgenic lines, overexpressinon phenotypes, overview | Oryza sativa Japonica Group |
physiological function | enzyme OsUGE1 plays an important role in carbohydrate partitioning to the cell wall in Oryza sativa | Oryza sativa Japonica Group |