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Literature summary for 5.1.3.2 extracted from

  • Suzuki, S.; Matsuzawa, T.; Nukigi, Y.; Takegawa, K.; Tanaka, N.
    Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast (2010), Microbiology, 156, 708-718.
    View publication on PubMed
Search for more literature for 5.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
gene uge1, is constitutively expressed, recombinant expression in Escherichia coli strain XL-1 blue Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
additional information construction of disruption mutants, DELTAuge1 and DELTAuge1DELTAgal10. Both mutant strains are sensitive to hygromycin B. Oligosaccharide content is reduced in acid phosphatase prepared from the uge1DELTA strain. The uge1DELTA strain grown in 0.1% glucose, 2% galactose medium shows a rise of UDP-glucose/-galactose epimerase activity while no detectable increase in activity is observed in the uge1Dgal10D strain. Gal10p can replace loss of Uge1p in the uge1DELTA mutant in glucose medium. Growth and galactosylation phenotypes of uge1DELTA, overview Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schizosaccharomyces pombe Gal10p, a Gal-1-P uridylyltransferase, also shows UDP-glucose/-galactose 4-epimerase activity, overview ?
-
 ?
additional information Schizosaccharomyces pombe ARC039 Gal10p, a Gal-1-P uridylyltransferase, also shows UDP-glucose/-galactose 4-epimerase activity, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe Q9Y7X5 gene uge1; gene UGE1
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Schizosaccharomyces pombe ARC039 Q9Y7X5 gene uge1; gene UGE1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Gal10p, a Gal-1-P uridylyltransferase, also shows UDP-glucose/-galactose 4-epimerase activity, overview Schizosaccharomyces pombe ?
-
 ?
additional information Uge1 protein contains only one epimerase domain Schizosaccharomyces pombe ?
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 ?
additional information Gal10p, a Gal-1-P uridylyltransferase, also shows UDP-glucose/-galactose 4-epimerase activity, overview Schizosaccharomyces pombe ARC039 ?
-
 ?
additional information Uge1 protein contains only one epimerase domain Schizosaccharomyces pombe ARC039 ?
-
 ?

Synonyms

Synonyms Comment Organism
UDP-glucose/-galactose 4-epimerase
-
 Schizosaccharomyces pombe
Uge1p
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 Schizosaccharomyces pombe

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
 assay at Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Schizosaccharomyces pombe

Expression

Organism Comment Expression
Schizosaccharomyces pombe uge1 is not induced by glucose additional information