Literature summary for 5.1.3.2 extracted from

Barber, C.; Roesti, J.; Rawat, A.; Findlay, K.; Roberts, K.; Seifert, G.J.
Distinct properties of the five UDP-D-glucose/UDP-D-galactose 4-epimerase isoforms of Arabidopsis thaliana (2006), J. Biol. Chem., 281, 17276-17285.

Search for more literature for 5.1.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and Saccharomyces cerevisiae	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	inhibited by combination of 100 microM NADH and 10 microM NAD+	Arabidopsis thaliana
NaCl	-	Arabidopsis thaliana
NADH	slightly inhibiting	Arabidopsis thaliana
NADPH	very weak inhibitory effect	Arabidopsis thaliana
UDP	-	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	UDP-glucose	values are indirectly determined from the Haldane relationship	Arabidopsis thaliana
0.1	0.13	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana
0.13	-	UDP-glucose	values are indirectly determined from the Haldane relationship	Arabidopsis thaliana
0.17	0.22	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana
0.19	-	UDP-glucose	values are indirectly determined from the Haldane relationship	Arabidopsis thaliana
0.19	0.22	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana
0.23	0.3	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana
0.56	-	UDP-glucose	values are indirectly determined from the Haldane relationship	Arabidopsis thaliana
0.76	-	UDP-glucose	values are indirectly determined from the Haldane relationship	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Arabidopsis thaliana	5737	-
Golgi apparatus	-	Arabidopsis thaliana	5794	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	increases the activity	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	gel filtration	Arabidopsis thaliana
40200	-	gel filtration	Arabidopsis thaliana
40300	-	gel filtration	Arabidopsis thaliana
40800	-	gel filtration	Arabidopsis thaliana
41100	-	gel filtration	Arabidopsis thaliana
84000	-	Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model.	Arabidopsis thaliana
91000	-	Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model.	Arabidopsis thaliana
98000	-	Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model.	Arabidopsis thaliana
103000	-	Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model.	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-D-glucose	Arabidopsis thaliana	carbohydrate biosynthesis	UDP-D-galactose	-	r
UDP-D-glucose	Arabidopsis thaliana	carbohydrate catabolism	UDP-D-galactose	-	r

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-D-glucose	carbohydrate biosynthesis	Arabidopsis thaliana	UDP-D-galactose	-	r
UDP-D-glucose	carbohydrate catabolism	Arabidopsis thaliana	UDP-D-galactose	-	r

Subunits

Subunits	Comment	Organism
dimer	-	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
UDP-D-glucose/UDP-D-galactose 4-epimerase	-	Arabidopsis thaliana
UGE1	-	Arabidopsis thaliana
UGE2	-	Arabidopsis thaliana
UGE3	-	Arabidopsis thaliana
UGE4	-	Arabidopsis thaliana
UGE5	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Arabidopsis thaliana
35	-	-	Arabidopsis thaliana
40	-	-	Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	40	-	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	8	UDP-glucose	The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM).	Arabidopsis thaliana
19	-	UDP-glucose	The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM).	Arabidopsis thaliana
23	24	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana
24	-	UDP-glucose	The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM).	Arabidopsis thaliana
28	34	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana
32	-	UDP-glucose	The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM).	Arabidopsis thaliana
33	-	UDP-glucose	The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM).	Arabidopsis thaliana
42	66	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana
89	101	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana
115	128	UDP-galactose	Different enzyme concentrations give slight variations in activity.	Arabidopsis thaliana

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	7	MES buffer	Arabidopsis thaliana
6.5	9.5	Tris/HCl buffer	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Arabidopsis thaliana
NAD+	is particularly important	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)