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Literature summary for 5.1.3.2 extracted from
- Biochemical characterization of UDP-GlcNAc/Glc 4-epimerase from Escherichia coli O86:B7 (2006), Biochemistry, 45, 13760-13768.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S306Y | plasmid containing the Gne S306Y constructed using the QuikChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA).The S306Y mutation totally abolished activity toward the acetylated substrate. | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.295 | - |
UDP-Gal | - |
Escherichia coli |  |
| 0.323 | - |
UDP-GlcNAc | - |
Escherichia coli | |
| 0.37 | - |
UDP-Glc | - |
Escherichia coli | |
| 0.373 | - |
UDP-GalNAc | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38800 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-Glc | Escherichia coli | - |
UDP-Gal | - |
r | |
| UDP-Glc | Escherichia coli O86:B7 | - |
UDP-Gal | - |
r | |
| UDP-GlcNAc | Escherichia coli | - |
UDP-GalNAc | - |
r | |
| UDP-GlcNAc | Escherichia coli O86:B7 | - |
UDP-GalNAc | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
O86:B7 | - |
| Escherichia coli O86:B7 | - |
O86:B7 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-Glc | - |
Escherichia coli | UDP-Gal | - |
r | |
| UDP-Glc | - |
Escherichia coli O86:B7 | UDP-Gal | - |
r | |
| UDP-GlcNAc | - |
Escherichia coli | UDP-GalNAc | - |
r | |
| UDP-GlcNAc | - |
Escherichia coli O86:B7 | UDP-GalNAc | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38800, gel filtration | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GNE | - |
Escherichia coli |
| UDP-GlcNAc/Glc 4-epimerase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | - |
- |
6.8 |
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