Literature summary for 5.1.3.19 extracted from

Tykesson, E.; Mao, Y.; Maccarana, M.; Pu, Y.; Gao, J.; Lin, C.; Zaia, J.; Westergren-Thorsson, G.; Ellervik, U.; Malmstroem, L.; Malmstroem, A.
Deciphering the mode of action of the processive polysaccharide modifying enzyme dermatan sulfate epimerase 1 by hydrogen-deuterium exchange mass spectrometry (2016), Chem. Sci., 7, 1447-1456 .

Search for more literature for 5.1.3.19

Cloned(Commentary)

Cloned (Comment)	Organism
functional recombinant enzyme expression in HEK-293 cells, all predicted N-glycans in DS-epi1 are present when the protein is expressed in the cells	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Chondroitin D-glucuronate	Homo sapiens	-	Dermatan L-iduronate	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	predicted N-glycans in DS-epi1 that are necessary for optimal enzymatic activity	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
Chondroitin D-glucuronate = dermatan L-iduronate	processive reducing to non-reducing end mode of action of DS-epi1, in silico modeling of site-specific information	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Chondroitin D-glucuronate	-	Homo sapiens	Dermatan L-iduronate	-	r
additional information	tandem mass spectrometry analysis, based on fragment analysis of reducing-end labeled GAG oligosaccharides, of heterogeneous epimerase products, DS-epi1-catalyzed incorporation of deuterium into the oligosaccharide substrates causes a discernible shift of the isotopic pattern in the mass spectra, and generation of site-specific modification information by acid-catalyzed glycan sequencing (PRAGS). Optimal substrate binding requires an octasaccharide or longer	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
dermatan sulfate epimerase 1	-	Homo sapiens
DS-epi1	-	Homo sapiens
DSE	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
additional information	polysaccharide processivity opens up the possibility for an efficient formation of long stretches of IdoA, which have been shown to be of major importance for CS/DS regulatory effect on collagen fibrillization	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)