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Literature summary for 5.1.3.19 extracted from
- Two dermatan sulfate epimerases form iduronic acid domains in dermatan sulfate (2009), J. Biol. Chem., 284, 9788-9795.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and genomic structures of DS-epi1 and DS-epi2, transient functional overexpression of myc/His-tagged DS-epi1 and FLAG-tagged DS-epi2 in HEK-293 cell microsomes | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | siRNA gene silencing of DS-epi2 and DS-epi1 in lung HFL-1 fibroblasts | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucuronate | Homo sapiens | in dermatan sulfate, iduronic acid residues are either clustered together in blocks or alternating with glucuronic acid, forming hybrid structures | L-iduronate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant myc/His-tagged DS-epi1 and FLAG-tagged DS-epi2 partially in HEK-293 cell microsomes | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HFL-1 cell | - |
Homo sapiens | - |
| lung fibroblast | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucuronate | in dermatan sulfate, iduronic acid residues are either clustered together in blocks or alternating with glucuronic acid, forming hybrid structures | Homo sapiens | L-iduronate | - |
? | |
| D-glucuronate | DS-epi2 has epimerase activity, which involves conversion of D-glucuronic acid to L-iduronic acid, EC 5.1.3.19, but no O-sulfotransferase activity, ovcerview | Homo sapiens | L-iduronate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | domain structures of DS-epi1 and DS-epi2, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dermatan sulfate epimerase | - |
Homo sapiens |
| DS-epi1 | - |
Homo sapiens |
| DS-epi2 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.5 | assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | DS-epi2 is genetically linked to bipolar disorder, which suggests that the dermatan sulfate domains generated by a defective enzyme may be involved in the etiology of the disease | Homo sapiens |
| physiological function | DS-epi2 and DS-epi1 are both involved in the biosynthesis of the iduronic acid blocks in fibroblasts and that DS-epi2 can also synthesize the hybrid structures | Homo sapiens |
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Release 2023.1 (January 2023)
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