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Literature summary for 5.1.3.17 extracted from
- Biosynthesis of heparin/heparan sulfate. Purification of the D-glucuronyl C-5 epimerase from bovine liver (1994), J. Biol. Chem., 269, 26953-26958.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | stimulates, maximal effect at 0.1 M, inhibition at higher concentrations | Bos taurus |  |
| NaCl | stimulates, maximal effect at 0.05 M, inhibition at higher concentrations | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
uronic acid residues | - |
Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no specific metal ion requirement | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, liver, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bos taurus | enzyme is involved in the biosynthesis of heparan sulfate in liver | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10% glycerol, retains full activity for 1.5 years | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Heparosan-N-sulfate D-glucuronate | heparosan N-sulfate prepared by metabolic labeling of a capsular polysaccharide from E. coli K5 and subsequent chemical partial N-deacetylation and N-sulfation. Approximately 30% of the D-glucuronyl residues located between two N-sulfated glucosamine units are converted to L-iduronyl units | Bos taurus | Heparosan-N-sulfate L-iduronate | - |
? | |
| additional information | enzyme is involved in the biosynthesis of heparan sulfate in liver | Bos taurus | ? | - |
? | |
| uronic acid residues | - |
Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, liver, SDS-PAGE | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 620 | - |
uronic acid residues | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | 7.9 | pH 6.1: about 60% of maximal activity, pH 7.9: about 30% of maximal activity | Bos taurus |
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Release 2023.1 (January 2023)
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