Literature summary for 5.1.3.14 extracted from

Namboori, S.C.; Graham, D.E.
Acetamido sugar biosynthesis in the Euryarchaea (2008), J. Bacteriol., 190, 2987-2996.

Search for more literature for 5.1.3.14

Activating Compound

Activating Compound	Comment	Organism	Structure
KCl	200 mM, twofold stimualtion	Methanococcus maripaludis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, C-terminal His-tag	Methanococcus maripaludis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Hill coefficient of 2.7, positive cooperativity	Methanococcus maripaludis
0.36	-	UDP-N-acetyl-D-glucosamine	pH 8.5, 37°C	Methanococcus maripaludis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme	Methanococcus maripaludis
42400	-	2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme	Methanococcus maripaludis
101000	-	gel filtration	Methanococcus maripaludis

Organism

Organism	UniProt	Comment	Textmining
Methanococcus maripaludis	Q6LZC4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine	-	Methanococcus maripaludis	UDP-N-acetyl-D-mannosamine	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme	Methanococcus maripaludis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Methanococcus maripaludis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.4	-	UDP-N-acetyl-D-glucosamine	pH 8.5, 37°C	Methanococcus maripaludis

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	-	Methanococcus maripaludis

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Release 2023.1 (January 2023)