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Literature summary for 5.1.3.14 extracted from

  • Reinke, S.O.; Eidenschink, C.; Jay, C.M.; Hinderlich, S.
    Biochemical characterization of human and murine isoforms of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) (2009), Glycoconj. J., 26, 415-422.
    View publication on PubMed
Search for more literature for 5.1.3.14

Cloned(Commentary)

Cloned (Comment) Organism
splice variant hGNE2, expression in insect and mammalian cells. Splice variant hGNE3, expression in Escherichia coli Homo sapiens
splice variants mGNE1, mGNE2, mGNE3 Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information splice variant hGNE2, recombinantly expressed in insect and mamalian cells, displays selective reduction of UDPGlcNAc 2-epimerase activity by the loss of its tetrameric state, which is essential for full enzyme activity. Splice variant hGNE3 only possesses kinase activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens role of splice variant GNE1 in basic supply of cells with sialic acids, whereas GNE2 and GNE3 may have a function in finetuning of the sialic acid pathway ?
-
 ?
additional information Mus musculus role of splice variant GNE1 in basic supply of cells with sialic acids, whereas GNE2 and GNE3 may have a function in finetuning of the sialic acid pathway. No significant differences in activities of splice variants mGNE 1 and mGNE2 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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 bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, splice variant hGNE2
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Mus musculus
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 bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, splice variant hGNE2
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information role of splice variant GNE1 in basic supply of cells with sialic acids, whereas GNE2 and GNE3 may have a function in finetuning of the sialic acid pathway Homo sapiens ?
-
 ?
additional information role of splice variant GNE1 in basic supply of cells with sialic acids, whereas GNE2 and GNE3 may have a function in finetuning of the sialic acid pathway. No significant differences in activities of splice variants mGNE 1 and mGNE2 Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
dimer recombinant splice variant hGNE1, mixture of tetramers and dimers in ratio of 3:1. Recombinant splice variant hGNE2, dimer. Gel filtration expreriments Homo sapiens
dimer recombinant splice variant mGNE1, mixture of tetramers and dimers in ratio of 4:1. Gel filtration expreriments Mus musculus
tetramer recombinant splice variant hGNE1, mixture of tetramers and dimers in ratio of 3:1, gel filtration Homo sapiens
tetramer recombinant splice variant mGNE1, mixture of tetramers and dimers in ratio of 4:1. Splice variant mGNE2, fully tetrameric state, gel filtration expreriments Mus musculus