Literature summary for 5.1.3.14 extracted from

Blume, A.; Weidemann, W.; Stelzl, U.; Wanker, E.E.; Lucka, L.; Donner, P.; Reutter, W.; Horstkorte, R.; Hinderlich, S.
Domain-specific characteristics of the bifunctional key enzyme of sialic acid biosynthesis, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (2004), Biochem. J., 384, 599-607.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Sf9 insect cells, wild-type enzyme and mutant enzymes DELTA1-39, DELTA1-234, DELTA1-356, DELTA383-722, DELTA490-722, DELTA597-722, DELTA697-722, DELTA717-722	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
DELTA1-234	mutant enzyme shows no N-epimerase activity	Rattus norvegicus
DELTA1-356	mutant enzyme shows no N-epimerase activity	Rattus norvegicus
DELTA1-39	mutant enzyme shows no N-epimerase activity	Rattus norvegicus
DELTA383-722	epimerase activity is 2% of wild-type enzyme	Rattus norvegicus
DELTA490-722	epimerase activity is 15% of wild-type enzyme	Rattus norvegicus
DELTA597-722	epimerase activity is 2% of wild-type enzyme	Rattus norvegicus
DELTA697-722	epimerase activity is about 70% of wild-type enzyme	Rattus norvegicus
DELTA717-722	epimerase activity is about 95% of wild-type enzyme	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
85000	-	6 * 85000, SDS-PAGE	Rattus norvegicus
500000	-	gel filtration	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Subunits

Subunits	Comment	Organism
hexamer	6 * 85000, SDS-PAGE	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	bifunctional enzyme	Rattus norvegicus

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Release 2023.1 (January 2023)