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Literature summary for 5.1.3.13 extracted from

  • Singh, B.; Oh, T.; Sohng, J.
    Exploration of two epimerase homologs in Streptomyces peucetius ATCC 27952 (2013), Appl. Microbiol. Biotechnol., 97, 2493-2502.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene dnmU, DNA and amino acid sequence determination, analysis, and comparison, genetic organization, overview. Recombinant expression in Escherichia coli Streptomyces peucetius
gene rmbC, DNA and amino acid sequence determination, analysis, and comparison, genetic organization, overview. Recombinant expression in Escherichia coli Streptomyces peucetius

Protein Variants

Protein Variants Comment Organism
additional information generation of rmbC-defective mutant Streptomyces peucetius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-4-dehydro-6-deoxy-alpha-D-glucose Streptomyces peucetius
-
dTDP-4-dehydro-beta-L-rhamnose
-
?
dTDP-4-dehydro-6-deoxy-alpha-D-glucose Streptomyces peucetius ATCC 27952
-
dTDP-4-dehydro-beta-L-rhamnose
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces peucetius C5J048 gene rmbC
-
Streptomyces peucetius O33707 gene dnmU
-
Streptomyces peucetius ATCC 27952 C5J048 gene rmbC
-
Streptomyces peucetius ATCC 27952 O33707 gene dnmU
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Streptomyces peucetius
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
-
Streptomyces peucetius dTDP-4-dehydro-beta-L-rhamnose
-
?
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
-
Streptomyces peucetius ATCC 27952 dTDP-4-dehydro-beta-L-rhamnose
-
?

Synonyms

Synonyms Comment Organism
deoxythymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase
-
Streptomyces peucetius
DnmU
-
Streptomyces peucetius
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
-
Streptomyces peucetius
RmbC
-
Streptomyces peucetius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptomyces peucetius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Streptomyces peucetius

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Streptomyces peucetius
NADH
-
Streptomyces peucetius

General Information

General Information Comment Organism
malfunction disruption of rmbC results in fragmented mycelia that quickly convert into gray pigmented spores Streptomyces peucetius
metabolism the enzyme from the doxorubicin biosynthesis gene cluster is involved in the biosynthesis of dTDP-L-daunosamine, pathway overview. It does not compensate for the other dTDP-4-keto-6-deoxyglucose 3,5-epimerase encoded by gene rmbC. Although dnmU and rmbC encode for similar functional proteins, their native roles in their respective biosynthetic pathways in vivo are specific and independent of one other Streptomyces peucetius
metabolism the enzyme is involved in the biosynthesis of dTDP-L-rhamnose, a precursor of cell wall biosynthesis, pathway overview. It does not compensate for the other dTDP-4-keto-6-deoxyglucose 3,5-epimerase encoded by gene dnmU. Although dnmU and rmbC encode for similar functional proteins, their native roles in their respective biosynthetic pathways in vivo are specific and independent of one other Streptomyces peucetius