BRENDA - Enzyme Database
show all sequences of 5.1.3.13

Biosynthesis of streptomycin. dTDP-dihydrostreptose synthase from Streptomyces griseus and dTDP-4-keto-L-rhamnose 3,5-epimerase from S. griseus and Escherichia coli Y10

Wahl, H.P.; Grisebach, H.; Biochim. Biophys. Acta 568, 243-252 (1979)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
dTDP-4-dehydro-6-deoxy-D-glucose
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
gel filtration
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli Y10
-
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose
dTDP-4-keto-L-rhamnose is formed as intermediate
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dTDP-4-dehydro-6-deoxy-D-glucose
-
2252
Escherichia coli
dTDP-4-dehydro-6-deoxy-L-mannose
-
2252
Escherichia coli
?
dTDP-4-dehydro-6-deoxy-D-glucose
-
2252
Escherichia coli Y10
dTDP-4-dehydro-6-deoxy-L-mannose
-
2252
Escherichia coli Y10
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
dTDP-4-dehydro-6-deoxy-D-glucose
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
gel filtration
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dTDP-4-dehydro-6-deoxy-D-glucose
-
2252
Escherichia coli
dTDP-4-dehydro-6-deoxy-L-mannose
-
2252
Escherichia coli
?
dTDP-4-dehydro-6-deoxy-D-glucose
-
2252
Escherichia coli Y10
dTDP-4-dehydro-6-deoxy-L-mannose
-
2252
Escherichia coli Y10
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Escherichia coli
Other publictions for EC 5.1.3.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746670
Shornikov
Structure of the Bacillus ant ...
Bacillus anthracis, Bacillus anthracis Ames
Acta Crystallogr. Sect. F
73
664-671
2017
-
-
1
1
-
-
-
-
-
-
-
2
-
11
-
-
1
-
-
-
-
-
2
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
747027
Feng
Identification of a dTDP-rham ...
Caenorhabditis elegans
Biochem. J.
473
1507-1521
2016
-
-
2
-
1
-
-
-
-
-
-
2
-
6
-
-
2
-
-
4
-
-
3
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
2
-
4
-
-
3
-
1
-
-
-
-
-
-
-
-
3
3
-
-
-
749151
Kaminski
Haloferax volcanii N-glycosyl ...
Haloferax volcanii, Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
PLoS ONE
9
e97441
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
14
-
-
1
-
-
-
-
-
2
-
4
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
726772
Singh
Exploration of two epimerase h ...
Streptomyces peucetius, Streptomyces peucetius ATCC 27952
Appl. Microbiol. Biotechnol.
97
2493-2502
2013
-
-
2
-
1
-
-
-
-
-
-
4
-
4
-
-
-
-
-
2
-
-
4
-
6
2
-
-
-
2
-
-
4
-
-
-
-
-
2
4
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
4
-
2
-
-
-
2
-
-
-
-
3
3
-
-
-
702695
Sivendran
Identification of triazinoindo ...
Mycobacterium tuberculosis
Bioorg. Med. Chem.
18
896-908
2010
-
-
-
-
-
-
10
1
1
1
-
1
-
4
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
9
10
-
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
703046
Nguyen
-
Spontaneous mutation results i ...
Komagataeibacter xylinus
Carbohydr. Polym.
80
338-344
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
715361
Tang
Glycosylation of the collagen ...
Aggregatibacter actinomycetemcomitans
J. Bacteriol.
192
1395-1404
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
716065
Parakkottil Chothi
Identification of an L-rhamnos ...
Acanthamoeba polyphaga Mimivirus
J. Virol.
84
8829-8838
2010
-
-
1
-
-
-
-
-
-
1
2
-
-
1
-
-
-
-
-
-
-
-
1
1
2
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
675420
Dong
RmlC, a C3 and C5 carbohydrate ...
Salmonella enterica, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
365
146-159
2007
-
3
2
2
3
-
-
3
-
-
3
4
-
183
-
-
-
-
-
-
-
-
4
-
4
-
-
-
4
-
-
-
3
-
-
-
-
3
2
3
2
3
-
-
-
-
3
-
-
3
4
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
660809
Thuy
Functional characterizations o ...
Streptomyces niveus
Arch. Biochem. Biophys.
436
161-167
2005
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661486
Elling
An enzyme module system for th ...
Salmonella enterica
ChemBioChem
6
1423-1430
2005
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653912
Dong
High-resolution structures of ...
Streptococcus suis
Structure
11
715-723
2003
-
1
-
1
-
-
-
1
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
652025
Christendat
Crystal structure of dTDP-4-ke ...
Methanothermobacter thermautotrophicus
J. Biol. Chem.
275
24608-24612
2000
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653311
Giraud
RmlC, the third enzyme of dTDP ...
Salmonella enterica
Nat. Struct. Biol.
7
398-402
2000
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285846
Graninger
Characterization of dTDP-4-deh ...
Salmonella enterica
J. Biol. Chem.
274
25069-25077
1999
-
-
1
-
-
-
-
1
-
-
1
1
-
4
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2255
Otten
Cloning and characterization o ...
Streptomyces peucetius
J. Bacteriol.
179
4446-4450
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2256
Mamuro
Enzymatic synthesis and isolat ...
Escherichia coli
Eur. J. Biochem.
204
539-545
1992
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2252
Wahl
Biosynthesis of streptomycin. ...
Escherichia coli, Escherichia coli Y10
Biochim. Biophys. Acta
568
243-252
1979
-
-
-
-
-
-
-
1
-
-
1
-
-
8
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2253
Wahl
Two enzymes in Streptomyces gr ...
Streptomyces griseus
Biochem. Biophys. Res. Commun.
64
1041-1045
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2254
Gaugler
Biological mechanisms involved ...
Escherichia coli, Pseudomonas aeruginosa, Escherichia coli 45
J. Biol. Chem.
248
6041-6049
1973
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-