Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli | Dictyoglomus turgidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
75.8 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
79.6 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
recombinant His-tagged enzyme, gel filtration | Dictyoglomus turgidum |
47200 | - |
1 * 47000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 47200, about, recombinant His-tagged enzyme, sequence calculation | Dictyoglomus turgidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose | Dictyoglomus turgidum | - |
D-glucosyl-D-mannose | - |
? | |
cellobiose | Dictyoglomus turgidum DSM 6724 | - |
D-glucosyl-D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyoglomus turgidum | - |
- |
- |
Dictyoglomus turgidum DSM 6724 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 10fold from Escherichia coli by heat treatment and metal affinity chromatography | Dictyoglomus turgidum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.2 | - |
purified recombinant His-tagged enzyme, substrate lactose, pH 7.0, 70°C | Dictyoglomus turgidum |
16.8 | 24.4 | purified recombinant His-tagged enzyme, substrate cellolbiose, pH 7.0, 70°C | Dictyoglomus turgidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose | - |
Dictyoglomus turgidum | 4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
cellobiose | - |
Dictyoglomus turgidum DSM 6724 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
cellobiose | - |
Dictyoglomus turgidum | D-glucosyl-D-mannose | - |
? | |
cellobiose | - |
Dictyoglomus turgidum DSM 6724 | D-glucosyl-D-mannose | - |
? | |
cellotriose | - |
Dictyoglomus turgidum | 4-O-beta-D-glucopyranosyl-4-O-beta-D-glucopyranosyl-D-mannose | - |
? | |
D-glucose | - |
Dictyoglomus turgidum | D-mannose | - |
? | |
D-glucose | - |
Dictyoglomus turgidum DSM 6724 | D-mannose | - |
? | |
lactose | - |
Dictyoglomus turgidum | epilactose | - |
? | |
lactose | - |
Dictyoglomus turgidum DSM 6724 | epilactose | - |
? | |
lactose | - |
Dictyoglomus turgidum | lactulose | - |
? | |
maltose | - |
Dictyoglomus turgidum | 4-O-alpha-D-glucopyranosyl-D-mannose | - |
? | |
maltotriose | - |
Dictyoglomus turgidum | 4-O-alpha-D-glucopyranosyl-4-O-alpha-D-glucopyranosyl-D-mannose | - |
? | |
additional information | the enzyme catalyzes not only epimerization but also isomerization for beta-1,4- and alpha-1,4-gluco-oligosaccharides. No activity with trehalose, sophorose, laminaribiose, gentiobiose, or xylobiose | Dictyoglomus turgidum | ? | - |
? | |
additional information | the enzyme catalyzes not only epimerization but also isomerization for beta-1,4- and alpha-1,4-gluco-oligosaccharides. No activity with trehalose, sophorose, laminaribiose, gentiobiose, or xylobiose | Dictyoglomus turgidum DSM 6724 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, recombinant His-tagged enzyme, SDS-PAGE, 1 * 47200, about, recombinant His-tagged enzyme, sequence calculation | Dictyoglomus turgidum |
Synonyms | Comment | Organism |
---|---|---|
cellobiose 2-epimerase | - |
Dictyoglomus turgidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Dictyoglomus turgidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | purified recombinant His-tagged enzyme, thermal inactivation of the cellobiose 2-epimerase follows first-order kinetics and the half-lives of the enzyme at 65°C, 70°C, 75°C, 80°C, and 85°C are 79 h, 55 h, 35 h, 20 h, and 4.8 h, respectively | Dictyoglomus turgidum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
89.4 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
169.1 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Dictyoglomus turgidum |
General Information | Comment | Organism |
---|---|---|
evolution | structure, active site, and reaction mechanism of cellobiose 2-epimerase are similar to those of N-acyl-D-glucosamine 2-epimerase | Dictyoglomus turgidum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
lactose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum | |
0.037 | - |
cellobiose | purified recombinant His-tagged enzyme, pH 7.0, 70°C | Dictyoglomus turgidum |