Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.3.1 extracted from

  • Lin, P.; Saha, R.; Zhang, F.; Pakrasi, H.
    Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocystis sp. PCC 6803 (2017), Sci. Rep., 7, 17503-17512 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.3.1

Application

Application Comment Organism
synthesis the enzyme overexpression is part of the engineering of Synechocystis 6803 for photosynthetic limonene production Synechocystis sp. PCC 6803

Cloned(Commentary)

Cloned (Comment) Organism
gene rpe, recombinant overexpression in Synechocystis sp. PCC 6803 Synechocystis sp. PCC 6803

Protein Variants

Protein Variants Comment Organism
additional information increased limonene production by genetic modifications: Two genes in the pentose phosphate pathway, rpi and rpe, are overexpressed in Synechocystis sp. PCC 6803 to divert carbon flux toward limonene production. The Abies grandis geranyl diphosphate synthase GPPS 2, that specifically produce geranyl diphosphate, is expressed to ensure sufficient geranyl diphosphate for limonene production. Codon-optimized limonene synthases from Mentha spicata and Citrus limon are heterologously expressed in Synechocystis sp. PCC 6803 to produce S-limonene and R-limonene, respectively, method overview and computational modeling Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Ribulose 5-phosphate Synechocystis sp. PCC 6803
-
 D-Xylulose 5-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 P74061
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ribulose 5-phosphate
-
 Synechocystis sp. PCC 6803 D-Xylulose 5-phosphate
-
 r

Synonyms

Synonyms Comment Organism
Ribulose 5-phosphate 3-epimerase
-
 Synechocystis sp. PCC 6803
RPE
-
 Synechocystis sp. PCC 6803