Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.2.6 extracted from

  • Hoshiko, S.; Kunimoto, Y.; Arima, K.; Beppu, T.
    Mechanism of L-alloisocitric acid fermentation: isocitrate epimerase activity in the cell-free extract of Penicillium purpurogenum (1982), Agric. Biol. Chem., 46, 143-151.
No PubMed abstract available
Search for more literature for 5.1.2.6

Activating Compound

Activating Compound Comment Organism Structure
pyridoxal 5'-phosphate slight stimulation Talaromyces purpureogenus

General Stability

General Stability Organism
30% v/v glycerol plus 1 mM DTT stabilize against heat inactivation, slight protection by leupeptin, pepstatin, and PMSF Talaromyces purpureogenus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Talaromyces purpureogenus 5739
-

Organism

Organism UniProt Comment Textmining
Talaromyces purpureogenus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Talaromyces purpureogenus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,2S)-1-Hydroxypropane-1,2,3-tricarboxylate i.e. threo-D-isocitrate Talaromyces purpureogenus (1S, 2S)-1-Hydroxypropane-1,2,3-tricarboxylate i.e. erythro-L-isocitrate ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 room temperature, half life: 12 h, with 30% glycerol, and 1 mM DTT, half-life: less than 3 h, without stabilizers Talaromyces purpureogenus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7 5.6 synthesis of erythro-isocitrate Talaromyces purpureogenus

pH Range

pH Minimum pH Maximum Comment Organism
3 8 3: about 35% of maximal activity, 8: about 30% of maximal activity, synthesis of erythro-isocitrate Talaromyces purpureogenus