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Literature summary for 5.1.2.2 extracted from
- Kinetics and thermodynamics of mandelate racemase catalysis (2002), Biochemistry, 41, 4048-4058.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sucrose | slight activating effect of sucrose on mutant enzyme efficiency. In presence of polymeric viscosogens poly(ethylene glycol) and Ficoll, no effect on turnover number or the ratio of turnover number and Km-value for the wild-type enzyme is observed | Pseudomonas putida |  |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N197A | slight activating effect of sucrose on mutant enzyme efficiency. In presewnce of polymeric viscosogens poly(ethylene glycol) and Ficoll, no effect on turnover number or the ratio of turnover number and Km-value for the wild-type enzyme is observed | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-mandelate | - |
Pseudomonas putida | (S)-mandelate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the turnover number of the mutant enzyme is unaffected by varying solvent viscosity | Pseudomonas putida |
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