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Literature summary for 5.1.2.2 extracted from
- Mandelate racemase (1979), Adv. Enzymol. Relat. Areas Mol. Biol., 50, 325-360.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-hydroxy-2-naphthoic acid | reversible | Pseudomonas putida |  |
| 3-Hydroxy-2-naphthoic acid | reversible | Pseudomonas putida | |
| 4-Hydroxycoumarin | reversible | Pseudomonas putida | |
| Anthranil-3-carboxylic acid | reversible | Pseudomonas putida | |
| Coumarilic acid | reversible | Pseudomonas putida | |
| DL-alpha-Phenylglycidate | - |
Pseudomonas putida | |
| EDTA | - |
Pseudomonas putida | |
| salicylate | reversible | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.63 | - |
D-mandelate | - |
Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement | Pseudomonas putida | |
| Co2+ | absolute requirement for a divalent metal ion | Pseudomonas putida | |
| Fe2+ | absolute requirement for a divalent metal ion: Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ | Pseudomonas putida | |
| Mg2+ | Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement | Pseudomonas putida | |
| Mg2+ | absolute requirement for a divalent metal ion | Pseudomonas putida | |
| Mn2+ | Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement | Pseudomonas putida | |
| Mn2+ | absolute requirement for a divalent metal ion | Pseudomonas putida | |
| Ni2+ | Mg2+, Mn2+, Ni2+, Co2+ or Fe2+ satisfy the divalent metal ion requirement | Pseudomonas putida | |
| Ni2+ | absolute requirement for a divalent metal ion | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69500 | - |
4 * 69500, SDS-PAGE | Pseudomonas putida |
| 278000 | - |
- |
Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mandelate | Pseudomonas putida | strictly inducible enzyme. Inducers: D-mandelate, L-mandelate, benzoylformate | L-mandelate | - |
? | |
| D-mandelate | Pseudomonas putida | first enzyme of pathway of oxidation of D,L-mandelate that permits certain strains of Pseudomonas putida to use mandelate as a sole source of carbon and energy | L-mandelate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
biotype A, ATCC 12633, this strain is also known as A.3.12 and PRS | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (S)-mandelate = (R)-mandelate | pathway involving a carbanion intermediate | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mandelate | - |
Pseudomonas putida | L-mandelate | - |
? | |
| D-mandelate | strictly inducible enzyme. Inducers: D-mandelate, L-mandelate, benzoylformate | Pseudomonas putida | L-mandelate | - |
? | |
| D-mandelate | first enzyme of pathway of oxidation of D,L-mandelate that permits certain strains of Pseudomonas putida to use mandelate as a sole source of carbon and energy | Pseudomonas putida | L-mandelate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 69500, SDS-PAGE | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1070 | - |
D-mandelate | - |
Pseudomonas putida | |
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