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Literature summary for 5.1.1.9 extracted from

  • Soda, K.; Yorifuji, T.; Ogata, K.
    Arginine racemase (Pseudomonas graveolens) (1976), Methods Enzymol., 17A, 341-345.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas graveolens

Inhibitors

Inhibitors Comment Organism Structure
D-Orn
-
Pseudomonas graveolens
hydroxylamine
-
Pseudomonas graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
pyridoxal 5'-phosphate cofactor pyridoxal 5'-phosphate Pseudomonas graveolens
1
-
D-Arg
-
Pseudomonas graveolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
167000
-
sedimentation equilibrium method Pseudomonas graveolens

Organism

Organism UniProt Comment Textmining
Pseudomonas graveolens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas graveolens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1250
-
-
Pseudomonas graveolens

Storage Stability

Storage Stability Organism
3-7°C, as a suspension in 0.02 M potassium phosphate buffer, pH 7.3, containing 0.1 mM pyridoxal 5'-phosphate and 60% saturated ammonium sulfate, stable for 6 months Pseudomonas graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg
-
Pseudomonas graveolens D-Arg
-
?
L-Citrulline 13% of the activity relative to L-Arg Pseudomonas graveolens D-Citrulline
-
?
L-Ethionine 13% of the activity relative to L-Arg Pseudomonas graveolens D-Ethionine
-
?
L-Glu 7% of the activity relative to L-Arg Pseudomonas graveolens D-Glu
-
?
L-Homoarginine 25% of the activity relative to L-Arg Pseudomonas graveolens D-Homoarginine
-
?
L-Homocitrulline 12% of the activity relative to L-Arg Pseudomonas graveolens D-Homocitrulline
-
?
L-Lys 110% of the activity relative to L-Arg Pseudomonas graveolens D-Lys
-
?
L-Met 4% of the activity relative to L-Arg Pseudomonas graveolens D-Met
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10 10.6
-
Pseudomonas graveolens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate contains 1 mol of firmly bound pyridoxal 5'-phosphate per 42000 g of protein Pseudomonas graveolens