Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas graveolens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Orn | - |
Pseudomonas graveolens | |
hydroxylamine | - |
Pseudomonas graveolens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
pyridoxal 5'-phosphate | cofactor pyridoxal 5'-phosphate | Pseudomonas graveolens | |
1 | - |
D-Arg | - |
Pseudomonas graveolens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
167000 | - |
sedimentation equilibrium method | Pseudomonas graveolens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas graveolens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas graveolens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1250 | - |
- |
Pseudomonas graveolens |
Storage Stability | Organism |
---|---|
3-7°C, as a suspension in 0.02 M potassium phosphate buffer, pH 7.3, containing 0.1 mM pyridoxal 5'-phosphate and 60% saturated ammonium sulfate, stable for 6 months | Pseudomonas graveolens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg | - |
Pseudomonas graveolens | D-Arg | - |
? | |
L-Citrulline | 13% of the activity relative to L-Arg | Pseudomonas graveolens | D-Citrulline | - |
? | |
L-Ethionine | 13% of the activity relative to L-Arg | Pseudomonas graveolens | D-Ethionine | - |
? | |
L-Glu | 7% of the activity relative to L-Arg | Pseudomonas graveolens | D-Glu | - |
? | |
L-Homoarginine | 25% of the activity relative to L-Arg | Pseudomonas graveolens | D-Homoarginine | - |
? | |
L-Homocitrulline | 12% of the activity relative to L-Arg | Pseudomonas graveolens | D-Homocitrulline | - |
? | |
L-Lys | 110% of the activity relative to L-Arg | Pseudomonas graveolens | D-Lys | - |
? | |
L-Met | 4% of the activity relative to L-Arg | Pseudomonas graveolens | D-Met | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 10.6 | - |
Pseudomonas graveolens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | contains 1 mol of firmly bound pyridoxal 5'-phosphate per 42000 g of protein | Pseudomonas graveolens |