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Literature summary for 5.1.1.8 extracted from
- Kinetic and structural studies of hydroxyproline 2-epimerase (1970), J. Biol. Chem., 245, 5248-5260.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Pyrrolidinol | - |
Pseudomonas putida |  |
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Pseudomonas putida | |
| pyrrole-2-carboxylate | - |
Pseudomonas putida | |
| sarcosine | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
2-2H-4-hydroxy-L-Pro | solvent H2O | Pseudomonas putida | |
| 13 | - |
4-hydroxy-L-Pro | solvent H2O | Pseudomonas putida | |
| 14 | - |
4-hydroxy-L-Pro | 2-2H-4-hydroxy-L-Pro | Pseudomonas putida | |
| 14 | - |
4-hydroxy-L-Pro | solvent 2H2O | Pseudomonas putida | |
| 19 | - |
3-allohydroxy-D-Pro | solvent 2H2O | Pseudomonas putida | |
| 24 | - |
2-2H-4-allohydroxy-D-Pro | solvent D2O | Pseudomonas putida | |
| 29 | - |
3-allohydroxy-L-Pro | solvent H2O | Pseudomonas putida | |
| 32 | - |
4-allohydroxy-L-Pro | solvent H2O | Pseudomonas putida | |
| 37 | - |
3-Hydroxy-L-Pro | solvent H2O | Pseudomonas putida | |
| 42 | - |
2-2H-4-allohydroxy-D-Pro | solvent H2O | Pseudomonas putida | |
| 49 | - |
4-allohydroxy-D-Pro | solvent H2O | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
ultracentrifugal data, study of tryptic peptides and amino acid composition, gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
wild-type and hyperinducible strain M-4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| trans-4-Hydroxy-L-proline = cis-4-hydroxy-D-proline | the alpha-hydrogen exchanges during enzymatic racemization at the alpha-carbon | Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:hydroxyproline-induced cell | - |
Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 154 | - |
hyperinducible strain M-4 | Pseudomonas putida |
| 164 | - |
wild type strain | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-allohydroxy-D-Pro | - |
Pseudomonas putida | 3-allohydroxy-L-Pro | - |
r | |
| 3-allohydroxy-L-Pro | - |
Pseudomonas putida | 3-allohydroxy-D-Pro | - |
r | |
| 3-hydroxy-L-Pro | - |
Pseudomonas putida | 3-hydroxy-D-Pro | - |
? | |
| Allohydroxy-D-Pro | - |
Pseudomonas putida | Allohydroxy-L-Pro | - |
? | |
| Hydroxy-L-Pro | - |
Pseudomonas putida | ? | - |
? | |
| trans-4-hydroxy-L-Pro | - |
Pseudomonas putida | cis-4-hydroxy-D-Pro | - |
? | |
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