Cloned (Comment) | Organism |
---|---|
gene dapF, overexpression of N-terminally His6-tagged enzyme, cloned with a a thrombin cleavage site, in Escherichia coli strain SoluBL21 | Acinetobacter baumannii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 14 mg/ml protein in 20 mM HEPES, pH 7.0, 100 mM NaCl, and 5 mM DTT, with 0.001 ml of reservoir solution containing 2 M ammonium sulfate, 0.1 M sodium HEPES, pH 7.5, and 4.9-5.1% PEG 400, equilibration against reservoir solution, 25% v/v PEG 400 as a cryoprotectant, method optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution | Acinetobacter baumannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31100 | - |
x * 31100 | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-Diaminoheptanedioate | Acinetobacter baumannii | - |
meso-Diaminoheptanedioate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
gene dapF | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain SoluBL21 by nickel affinity chromatography, tag cleavage by thrombin, another step of nickel affinity chromatography, ultrafiltration, and gel filtration | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-Diaminoheptanedioate | - |
Acinetobacter baumannii | meso-Diaminoheptanedioate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 31100 | Acinetobacter baumannii |
Synonyms | Comment | Organism |
---|---|---|
DapF | - |
Acinetobacter baumannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is independent of pyridoxal 5'-phosphate | Acinetobacter baumannii |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the pyridoxal 5'-phosphate-independent racemase family of enzymes | Acinetobacter baumannii |
metabolism | meso-diaminopimelate is a biosynthetic precursor of L-lysine in bacteria | Acinetobacter baumannii |
physiological function | (2R,6S)-2,6-diaminopimelic acid, i.e. meso-diaminopimelate, in the pentapeptide of cell wall peptidoglycan provides the attachment site for the inner or outer membrane to peptidoglycan | Acinetobacter baumannii |