Literature summary for 5.1.1.7 extracted from

Hor, L.; Dobson, R.; Dogovski, C.; Hutton, C.; Perugini, M.
Crystallization and preliminary X-ray diffraction analysis of diaminopimelate epimerase from Escherichia coli (2009), Acta Crystallogr. Sect. F, 66, 37-40.

Application

Application	Comment	Organism
drug development	DAP epimerase is an attractive target for rational antibiotic design	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
by using the sitting-drop vapour-diffusion method with droplets consisting of 150 nl protein solution and 150 nl reservoir solution, conditions that yield crystals are replicated using the hanging-drop vapourdiffusion method with drops containing 0.002 ml protein solution and 0.002 ml precipitant solution, crystals are obtained in space group P41212 and diffract to 2.0 A resolution, with unit-cell parameters a = b = 89.4, c = 179.6 A	Escherichia coli

Crystallization (Comment)

Organism

by using the sitting-drop vapour-diffusion method with droplets consisting of 150 nl protein solution and 150 nl reservoir solution, conditions that yield crystals are replicated using the hanging-drop vapourdiffusion method with drops containing 0.002 ml protein solution and 0.002 ml precipitant solution, crystals are obtained in space group P41212 and diffract to 2.0 A resolution, with unit-cell parameters a = b = 89.4, c = 179.6 A

Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31030	-	predicted, confirmed by SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
LL-2,6-diaminoheptanedioate	Escherichia coli	stereo-inversion	meso-diaminopimelate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by immobilized nickel metal-affinity chromatography using 5 ml HisTrap HP columns and by gel filtration as His-tagged enzyme	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.205	-	crude extract after expression	Escherichia coli
0.574	-	after immobilized metal-affinity chromatography	Escherichia coli
1.18	-	after gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
LL-2,6-diaminoheptanedioate	stereo-inversion	Escherichia coli	meso-diaminopimelate	-	?

Synonyms

Synonyms	Comment	Organism
DAP epimerase	-	Escherichia coli
diaminopimelate epimerase	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	enzyme is a member of the PLP-independent amino-acid racemases, it catalyzes the penultimate step of lysine biosynthesis in bacteria and plants	Escherichia coli