Cloned (Comment) | Organism |
---|---|
recombinant protein is expressed as His-tegged enzyme in BL21(DE3) Escherichia coli cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the ligand-free form refines to a resolution of 2.6 A, 2.5 mM dithiothreitol is present in the crystal drop | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-diaminoheptanedioate | Mycobacterium tuberculosis | stereo-inversion | meso-diaminopimelate | the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan | ? | |
LL-2,6-diaminoheptanedioate | Mycobacterium tuberculosis H37Rv | stereo-inversion | meso-diaminopimelate | the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WP19 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WP19 | - |
- |
Purification (Comment) | Organism |
---|---|
by using nickel-chelate affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-diaminoheptanedioate | stereo-inversion | Mycobacterium tuberculosis | meso-diaminopimelate | the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan | ? | |
LL-2,6-diaminoheptanedioate | stereo-inversion | Mycobacterium tuberculosis H37Rv | meso-diaminopimelate | the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan | ? |
Synonyms | Comment | Organism |
---|---|---|
DAP epimerase | - |
Mycobacterium tuberculosis |
DapF | - |
Mycobacterium tuberculosis |
diaminopimelate epimerase | - |
Mycobacterium tuberculosis |
MtDapF | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | synthesis of L-lysine | Mycobacterium tuberculosis |