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Literature summary for 5.1.1.4 extracted from

  • Chamond, N.; Gregoire, C.; Coatnoan, N.; Rougeot, C.; Freitas-Junior, L.H.; da Silveira, J.F.; Degrave, W.M.; Minoprio, P.
    Biochemical characterization of proline racemases from the human protozoan parasite Trypanosoma cruzi and definition of putative protein signatures (2003), J. Biol. Chem., 278, 15484-15494.
    View publication on PubMed
Search for more literature for 5.1.1.4

Application

Application Comment Organism
medicine potential of the enzyme as a critical target for drug development against Chagas‘ disease. The enzyme is absent in mammalian host, suggesting that inhibition of proline racemase may have therapeutic potential Trypanosoma cruzi
medicine potential of the enzyme as a critical target for drug development against Chaga‘s disease. The enzyme is absent in mammalian host, suggesting that inhibition of proline racemase may have therapeutic potential Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
pyrrole-2-carboxylic acid
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
29
-
 L-proline pH 6.0, 37°C Trypanosoma cruzi
75
-
 L-proline pH 6.0, 37°C Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular membrane-bound and secreted forms of enzyme are present upon differentiation of the parasite into non-dividing infective forms Trypanosoma cruzi
-
-
intracellular in replicative non-infective forms of Trypanosoma cruzi Trypanosoma cruzi 5622
-
membrane membrane-bound and secreted forms of enzyme are present upon differentiation of the parasite into non-dividing infective forms Trypanosoma cruzi 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40100
-
 2 * 40100, TcPRACB, SDS-PAGE Trypanosoma cruzi
45800
-
 2 * 45800, TcPRACA, SDS-PAGE Trypanosoma cruzi
80000
-
 TCPRACA, gel filtration, non-denaturing PAGE Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q4DA80
-
-
Trypanosoma cruzi Q868H8
-
-

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol or diluted in sodium acetate buffer, pH 6.0, activity is impaired Trypanosoma cruzi
-20°C, 50% glycerol or diluted in sodium acetate buffer, pH 6.0, stable Trypanosoma cruzi
-20°C, as ammonium sulfate precipitate, stable Trypanosoma cruzi
23°C, 0.5 M imidazole buffer, pH 8.0, 10 days, TcPRACA loses 84% of its activity Trypanosoma cruzi
23°C, 0.5 M imidazole buffer, pH 8.0, 10 days, TcPRACB is stable, TcPRACA loses 84% of its activity Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline
-
 Trypanosoma cruzi D-proline
-
 ?
additional information TcPRACB: no reaction with L-hydroxyproline Trypanosoma cruzi ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40100, TcPRACB, SDS-PAGE Trypanosoma cruzi
dimer 2 * 45800, TcPRACA, SDS-PAGE Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Trypanosoma cruzi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 5 min, activity is abolished Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 8.5
-
 Trypanosoma cruzi
5.5 7
-
 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
additional information TcPRACB is a cofactor-independent racemase Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0036
-
 pyrrole-2-carboxylic acid pH 6.0, 37°C, TcPRACB Trypanosoma cruzi
0.0057
-
 pyrrole-2-carboxylic acid pH 6.0, 37°C, TcPRACA Trypanosoma cruzi