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Literature summary for 5.1.1.3 extracted from

  • Ferla, M.P.; Brewster, J.L.; Hall, K.R.; Evans, G.B.; Patrick, W.M.
    Primordial-like enzymes from bacteria with reduced genomes (2017), Mol. Microbiol., 105, 508-524 .
    View publication on PubMed
Search for more literature for 5.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
gene metC, phylogenetic analysis, the recombinant His6-tagged enzyme TmCBL is overexpressed in a metC-deficient DELTAmetC Escherichia coli mutant strain and can complement it. The enzyme rescues the alanine racemase knockout, Escherichia coli strain MB2795 (DELTAalrDELTAdadX), as quickly as expressing Escherichia coli ALR itself. Recombinant production of His6-tagged enzyme in a soluble form Thermotoga maritima
gene metC, phylogenetic analysis, the recombinant His6-tagged enzyme wMelCBL is overexpressed in a metC-deficient DELTAmetC Escherichia coli mutant strain and can complement it. The enzyme rescues the alanine racemase knockout, Escherichia coli strain MB2795 (DELTAalrDELTAdadX), as quickly as expressing Escherichia coli ALR itself. Recombinant production of His6-tagged enzyme in a soluble form Wolbachia pipientis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten steady-state kinetics Wolbachia pipientis
additional information
-
 additional information Michaelis-Menten steady-state kinetics Thermotoga maritima
0.26
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer Thermotoga maritima
0.8
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 37°C Wolbachia pipientis
1.4
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Wolbachia pipientis
-
 D-glutamate
-
 r
L-glutamate Thermotoga maritima
-
 D-glutamate
-
 r
L-glutamate Wolbachia pipientis wMel
-
 D-glutamate
-
 r
L-glutamate Thermotoga maritima DSM 3109
-
 D-glutamate
-
 r
L-glutamate Thermotoga maritima ATCC 43589
-
 D-glutamate
-
 r
L-glutamate Thermotoga maritima JCM 10099
-
 D-glutamate
-
 r
additional information Wolbachia pipientis the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria ?
-
-
additional information Thermotoga maritima the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria ?
-
-
additional information Wolbachia pipientis wMel the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria ?
-
-
additional information Thermotoga maritima DSM 3109 the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria ?
-
-
additional information Thermotoga maritima ATCC 43589 the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria ?
-
-
additional information Thermotoga maritima JCM 10099 the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria ?
-
-

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X0Z7
-
-
Thermotoga maritima ATCC 43589 Q9X0Z7
-
-
Thermotoga maritima DSM 3109 Q9X0Z7
-
-
Thermotoga maritima JCM 10099 Q9X0Z7
-
-
Wolbachia pipientis Q73GL9 endosymbiont of Drosophila melanogaster
-
Wolbachia pipientis wMel Q73GL9 endosymbiont of Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Wolbachia pipientis D-glutamate
-
 r
L-glutamate
-
 Thermotoga maritima D-glutamate
-
 r
L-glutamate
-
 Wolbachia pipientis wMel D-glutamate
-
 r
L-glutamate
-
 Thermotoga maritima DSM 3109 D-glutamate
-
 r
L-glutamate
-
 Thermotoga maritima ATCC 43589 D-glutamate
-
 r
L-glutamate
-
 Thermotoga maritima JCM 10099 D-glutamate
-
 r
additional information the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria Wolbachia pipientis ?
-
-
additional information the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria Thermotoga maritima ?
-
-
additional information the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria Wolbachia pipientis wMel ?
-
-
additional information the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria Thermotoga maritima DSM 3109 ?
-
-
additional information the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria Thermotoga maritima ATCC 43589 ?
-
-
additional information the enzyme is a physiologically bifunctional alanine/glutamate racemase (EC 5.1.1.1/EC 5.1.1.3), it is not highly active, but it is clearly sufficient. The metC encoded L-alanine/L-glutamate racemase is a multifunctional CBL/ALR. CBL (EC 4.4.1.13) activity is no longer required in these bacteria Thermotoga maritima JCM 10099 ?
-
-

Synonyms

Synonyms Comment Organism
AAR
-
 Wolbachia pipientis
AAR
-
 Thermotoga maritima
CBL/ALR
-
 Wolbachia pipientis
CBL/ALR
-
 Thermotoga maritima
cystathionine beta-lyase
-
 Wolbachia pipientis
cystathionine beta-lyase
-
 Thermotoga maritima
GLR
-
 Thermotoga maritima
GRL
-
 Wolbachia pipientis
MetC
-
 Wolbachia pipientis
MetC
-
 Thermotoga maritima
More see also EC 4.4.1.13 and EC 5.1.1.1 Wolbachia pipientis
More see also EC 4.4.1.13 and EC 5.1.1.1 Thermotoga maritima
TmCBL
-
 Thermotoga maritima
wMelCBL
-
 Wolbachia pipientis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Wolbachia pipientis
70
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 90 activity range, profile overview Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 37°C Wolbachia pipientis
0.024
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer Thermotoga maritima
1
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Wolbachia pipientis
8
-
 assay at Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Wolbachia pipientis
pyridoxal 5'-phosphate
-
 Thermotoga maritima

General Information

General Information Comment Organism
evolution several bacteria with reduced genomes lack alr, encoding alanine racemase, but contain metC encoding cystathionine beta-lyase (CBL, EC 4.4.1.13), which, in these organisms, is a multifunctional CBL/ALR. CBL activity is no longer required in these bacteria. Primordial-like enzymes may be an essential part of the adaptive strategy associated with streamlining Wolbachia pipientis
evolution several bacteria with reduced genomes lack alr, encoding alanine racemase, but contain metC encoding cystathionine beta-lyase (CBL, EC 4.4.1.13), which, in these organisms, is a multifunctional CBL/ALR. CBL activity is no longer required in these bacteria. Primordial-like enzymes may be an essential part of the adaptive strategy associated with streamlining Thermotoga maritima
metabolism several bacteria with reduced genomes lack alr, encoding alanine racemase, but contain metC encoding cystathionine beta-lyase (CBL, EC 4.4.1.13), which, in these organisms, is a multifunctional CBL/ALR. CBL activity is no longer required in these bacteria Wolbachia pipientis
metabolism several bacteria with reduced genomes lack alr, encoding alanine racemase, but contain metC encoding cystathionine beta-lyase (CBL, EC 4.4.1.13), which, in these organisms, is a multifunctional CBL/ALR. CBL activity is no longer required in these bacteria Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.021
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 37°C Wolbachia pipientis
0.092
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer Thermotoga maritima
0.71
-
 L-glutamate recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer Thermotoga maritima