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Literature summary for 5.1.1.3 extracted from

  • Oh, S.Y.; Richter, S.G.; Missiakas, D.M.; Schneewind, O.
    Glutamate racemase mutants of Bacillus anthracis (2015), J. Bacteriol., 197, 1854-1861 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
identification of two insertions in gene racE1, suggesting that its glutamate racemase product is not essential for Bacillus anthracis growth Bacillus anthracis

Protein Variants

Protein Variants Comment Organism
additional information using allelic replacement, chromosomal racE2 is replaced with the spectinomycin resistance gene (aad9) and transduced via bacteriophage CP-51 into Bacillus anthracis strain Sterne, generating strain SYO2 (DELTAracE2::aad9) Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Bacillus anthracis
-
 D-glutamate
-
 r
L-glutamate Bacillus anthracis Sterne 34F2
-
 D-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81LA8
-
-
Bacillus anthracis Q81UL8
-
-
Bacillus anthracis Sterne 34F2 Q81LA8
-
-
Bacillus anthracis Sterne 34F2 Q81UL8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Bacillus anthracis D-glutamate
-
 r
L-glutamate
-
 Bacillus anthracis Sterne 34F2 D-glutamate
-
 r

Synonyms

Synonyms Comment Organism
BAS0806
-
 Bacillus anthracis
BAS4379
-
 Bacillus anthracis
GBAA_0847
-
 Bacillus anthracis
GBAA_4717
-
 Bacillus anthracis
MurI
-
 Bacillus anthracis
RacE1
-
 Bacillus anthracis
RacE2
-
 Bacillus anthracis

General Information

General Information Comment Organism
malfunction the racE1 deletion does not affect growth rates, whereas racE2 deletion delays the vegetative growth of Bacillus anthracis following spore germination, producing aberrant cell shapes that can only partially be suppressed with exogenous D-glutamate. Deletion of racE1 or racE2 does not affect the synthesis or stereochemical composition of Bacillus anthracis poly-gamma-D-glutamic acid (PDGA) capsule, and mutants with a deletion of both genes, racE1 and racE2, are not viable Bacillus anthracis
physiological function genes racE1 and racE2, encoding two glutamate racemases, are not essential for growth of Bacillus anthracis noncapsulating vaccine strain Sterne and fully virulent, capsulating strain Ames, even in the absence of exogenous D-glutamate. Modeling of Bacillus anthracis, similar to Bacillus subtilis, utilizing two functionally redundant racemase enzymes to synthesize D-glutamic acid for peptidoglycan synthesis Bacillus anthracis
physiological function genes racE1 and racE2, encoding two glutamate racemases,are not essential for growth of Bacillus anthracis noncapsulating vaccine strain Sterne and fully virulent, capsulating strain Ames, even in the absence of exogenous D-glutamate. Modeling of Bacillus anthracis, similar to Bacillus subtilis, utilizing two functionally redundant racemase enzymes to synthesize D-glutamic acid for peptidoglycan synthesis Bacillus anthracis