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Literature summary for 5.1.1.3 extracted from

  • Whalen, K.L.; Tussey, K.B.; Blanke, S.R.; Spies, M.A.
    Nature of allosteric inhibition in glutamate racemase: discovery and characterization of a cryptic inhibitory pocket using atomistic MD simulations and pKa calculations (2011), J. Phys. Chem. B, 115, 3416-3424.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus anthracis

Protein Variants

Protein Variants Comment Organism
K106A the isozyme RacE2 mutant is insensitive to dipicolinic acid Bacillus anthracis
S207A the isozyme RacE2 mutant is insensitive to dipicolinic acid Bacillus anthracis

Inhibitors

Inhibitors Comment Organism Structure
dipicolinic acid allosteric inhibitor of both Bacillus anthracis glutamate racemase isozymes which exhibits low micromolar inhibition with clear noncompetitive behavior Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Co2+-affinity column chromatography, and anion-exchange chromatography Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glutamate
-
Bacillus anthracis L-glutamate
-
?

Synonyms

Synonyms Comment Organism
RacE1 isozyme Bacillus anthracis
RacE2 isozyme Bacillus anthracis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus anthracis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.075
-
dipicolinic acid isozyme RacE1, pH and temperature not specified in the publication Bacillus anthracis
0.092
-
dipicolinic acid isozyme RacE2, pH and temperature not specified in the publication Bacillus anthracis
1.236
-
dipicolinic acid RacE2 mutant enzyme S207A, pH and temperature not specified in the publication Bacillus anthracis
2.342
-
dipicolinic acid RacE2 mutant enzyme K106A, pH and temperature not specified in the publication Bacillus anthracis