Application | Comment | Organism |
---|---|---|
drug development | glutamate racemase is an attractive target for the design of antibacterial agents | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
recombinant wild type and mutant proteins are expressed in Escherichia coli cells | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
T76A | production by site-directed mutagenesis, strong RacE-glutamate carbanion interaction energy is notably dissipated with the mutant | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-sulfobenzoic acid | structural analogue of dipicolinate dianion | Bacillus subtilis | |
dipicolinate dianion | DPA | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
D-glutamate | wild type enzyme, at 25 °C in 50 mM Tris-HCl (pH 8.0), 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate | Bacillus subtilis | |
8.6 | - |
D-glutamate | mutant T76A, at 25 °C in 50 mM Tris-HCl, pH 8.0, 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate | Bacillus subtilis | |
14 | - |
L-glutamate | wild type enzyme, 50 mM boric acid, 100 mM KCl, 0.2 mM dithiothreitol, pH 8.0 at 25°C in the presence of 0.22 microM glutamate racemase | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Bacillus subtilis | - |
D-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q6L876 | - |
- |
Purification (Comment) | Organism |
---|---|
by using nickel-chelate chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Bacillus subtilis | D-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
glutamate racemase | - |
Bacillus subtilis |
RACE | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
D-glutamate | mutant T76A, at 25°C in 50 mM Tris-HCl (pH 8.0), 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate | Bacillus subtilis | |
1.3 | - |
D-glutamate | wild type enzyme, at 25°C in 50 mM Tris-HCl, pH 8.0, 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate | Bacillus subtilis | |
87 | - |
L-glutamate | wild type enzyme, 50 mM boric acid, 100 mM KCl, 0.2 mM dithiothreitol, pH 8.0, at 25°C in the presence of 0.22 microM glutamate racemase | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.97 | - |
dipicolinate dianion | competitive inhibition | Bacillus subtilis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2.9 | - |
structural analogue of dipicolinate dianion | Bacillus subtilis | 3-sulfobenzoic acid |
General Information | Comment | Organism |
---|---|---|
physiological function | peptidoglycan biosynthesis | Bacillus subtilis |