Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces lavendulae subsp. lavendulae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-[[(carbamoylamino)oxy]methyl]oxiran-2-carboxylic acid | irreversible inhibition | Streptomyces lavendulae subsp. lavendulae | |
Hg2+ | 0.01 mM, complete inhibition | Streptomyces lavendulae subsp. lavendulae | |
iodoacetamide | complete inhibition | Streptomyces lavendulae subsp. lavendulae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
O-ureido-D-serine | pH 7.9, 30°C | Streptomyces lavendulae subsp. lavendulae | |
110 | - |
O-ureido-L-serine | pH 7.9, 30°C | Streptomyces lavendulae subsp. lavendulae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31205 | - |
x * 31205, ESI-TOF mass spectrometry | Streptomyces lavendulae subsp. lavendulae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-ureido-L-serine | Streptomyces lavendulae subsp. lavendulae | the enzyme is involved in the biosynthesis of the antibiotic D-cycloserine | O-ureido-D-serine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae subsp. lavendulae | D2Z026 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces lavendulae subsp. lavendulae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-ureido-L-serine = O-ureido-D-serine | the enzyme employs a two-base mechanism, with a thiolatethiol pair in the active site | Streptomyces lavendulae subsp. lavendulae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-ureido-L-serine | - |
Streptomyces lavendulae subsp. lavendulae | O-ureido-D-serine | - |
r | |
O-ureido-L-serine | the enzyme is involved in the biosynthesis of the antibiotic D-cycloserine | Streptomyces lavendulae subsp. lavendulae | O-ureido-D-serine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 31205, ESI-TOF mass spectrometry | Streptomyces lavendulae subsp. lavendulae |
Synonyms | Comment | Organism |
---|---|---|
DcsC | - |
Streptomyces lavendulae subsp. lavendulae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
O-ureido-D-serine | pH 7.9, 30°C | Streptomyces lavendulae subsp. lavendulae | |
158 | - |
O-ureido-L-serine | pH 7.9, 30°C | Streptomyces lavendulae subsp. lavendulae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Streptomyces lavendulae subsp. lavendulae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.44 | - |
O-ureido-L-serine | pH 7.9, 30°C | Streptomyces lavendulae subsp. lavendulae | |
1.71 | - |
O-ureido-D-serine | pH 7.9, 30°C | Streptomyces lavendulae subsp. lavendulae |