BRENDA - Enzyme Database
show all sequences of 5.1.1.19

Characterization of DcsC, a PLP-independent racemase involved in the biosynthesis of D-cycloserine

Dietrich, D.; van Belkum, M.J.; Vederas, J.C.; Org. Biomol. Chem. 10, 2248-2254 (2012) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Streptomyces lavendulae subsp. lavendulae
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-[[(carbamoylamino)oxy]methyl]oxiran-2-carboxylic acid
irreversible inhibition
Streptomyces lavendulae subsp. lavendulae
Hg2+
0.01 mM, complete inhibition
Streptomyces lavendulae subsp. lavendulae
iodoacetamide
complete inhibition
Streptomyces lavendulae subsp. lavendulae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
17
-
O-ureido-D-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
110
-
O-ureido-L-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31205
-
x * 31205, ESI-TOF mass spectrometry
Streptomyces lavendulae subsp. lavendulae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
O-ureido-L-serine
Streptomyces lavendulae subsp. lavendulae
the enzyme is involved in the biosynthesis of the antibiotic D-cycloserine
O-ureido-D-serine
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces lavendulae subsp. lavendulae
D2Z026
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Streptomyces lavendulae subsp. lavendulae
Reaction
Reaction
Commentary
Organism
Reaction ID
O-ureido-L-serine = O-ureido-D-serine
the enzyme employs a two-base mechanism, with a thiolate–thiol pair in the active site
Streptomyces lavendulae subsp. lavendulae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
O-ureido-L-serine
-
723316
Streptomyces lavendulae subsp. lavendulae
O-ureido-D-serine
-
-
-
r
O-ureido-L-serine
the enzyme is involved in the biosynthesis of the antibiotic D-cycloserine
723316
Streptomyces lavendulae subsp. lavendulae
O-ureido-D-serine
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 31205, ESI-TOF mass spectrometry
Streptomyces lavendulae subsp. lavendulae
Synonyms
Synonyms
Commentary
Organism
DcsC
-
Streptomyces lavendulae subsp. lavendulae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
29
-
O-ureido-D-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
158
-
O-ureido-L-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Streptomyces lavendulae subsp. lavendulae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces lavendulae subsp. lavendulae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-[[(carbamoylamino)oxy]methyl]oxiran-2-carboxylic acid
irreversible inhibition
Streptomyces lavendulae subsp. lavendulae
Hg2+
0.01 mM, complete inhibition
Streptomyces lavendulae subsp. lavendulae
iodoacetamide
complete inhibition
Streptomyces lavendulae subsp. lavendulae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
17
-
O-ureido-D-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
110
-
O-ureido-L-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31205
-
x * 31205, ESI-TOF mass spectrometry
Streptomyces lavendulae subsp. lavendulae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
O-ureido-L-serine
Streptomyces lavendulae subsp. lavendulae
the enzyme is involved in the biosynthesis of the antibiotic D-cycloserine
O-ureido-D-serine
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces lavendulae subsp. lavendulae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
O-ureido-L-serine
-
723316
Streptomyces lavendulae subsp. lavendulae
O-ureido-D-serine
-
-
-
r
O-ureido-L-serine
the enzyme is involved in the biosynthesis of the antibiotic D-cycloserine
723316
Streptomyces lavendulae subsp. lavendulae
O-ureido-D-serine
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 31205, ESI-TOF mass spectrometry
Streptomyces lavendulae subsp. lavendulae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
29
-
O-ureido-D-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
158
-
O-ureido-L-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Streptomyces lavendulae subsp. lavendulae
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.44
-
O-ureido-L-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
1.71
-
O-ureido-D-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.44
-
O-ureido-L-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
1.71
-
O-ureido-D-serine
pH 7.9, 30°C
Streptomyces lavendulae subsp. lavendulae
Other publictions for EC 5.1.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723316
Dietrich
Characterization of DcsC, a PL ...
Streptomyces lavendulae subsp. lavendulae
Org. Biomol. Chem.
10
2248-2254
2012
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1
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3
2
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1
1
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1
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1
1
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2
1
1
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2
1
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1
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3
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2
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1
1
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1
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2
1
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2
1
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2
2
721300
Kumagai
Molecular cloning and heterolo ...
Streptomyces lavendulae subsp. lavendulae
Antimicrob. Agents Chemother.
54
1132-1139
2010
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1
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1
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1
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1
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1
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1
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