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Literature summary for 5.1.1.18 extracted from

  • Talukdar, G.; Inoue, R.; Yoshida, T.; Ishimoto, T.; Yaku, K.; Nakagawa, T.; Mori, H.
    Novel role of serine racemase in anti-apoptosis and metabolism (2017), Biochim. Biophys. Acta, 1861, 3378-3387 .
    View publication on PubMed
Search for more literature for 5.1.1.18

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged wild-type and mutant enzymes in HEK-293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
K56G site-directed mutagenesis, catalytically inactive mutant Mus musculus
Q155D site-directed mutagenesis, catalytically hyperactive mutant, the mutant shows enhanced racemization and reduced alpha,beta-elimination activities Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Mus musculus 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Mus musculus
-
 D-serine
-
 r

Organism

Organism UniProt Comment Textmining
Mus musculus Q9QZX7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte
-
 Mus musculus
-
brain
-
 Mus musculus
-
neuron
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
 Mus musculus D-serine
-
 r
additional information the enzyme has two enzymatic activities, namely, racemization and alpha,beta-elimination Mus musculus ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mus musculus

General Information

General Information Comment Organism
malfunction HEK-293T cells expressing the wild-type enzyme and hyperactive mutant Q155D show resistance to staurosporine-induced apoptosis, compared with nontransfected HEK-293T cells and cells expressing the catalytically-dead enzyme mutant K56G. The wild-type enzyme-expressing cells also show a significant higher viability than the cells expressing hyperactive mutant Q155D enzyme mutant. Elevated phosphorylation levels of Bcl-2 at Ser70 and Akt at Ser473 and Thr308, which are related to cell survival, occur in the cells expressing wild-type enzyme and mutant Q155D, elevated levels of acetyl CoA and ATP in cells expressing the wild-type enzyme. Phenotypes, overview Mus musculus
physiological function serine racemase catalyzes the production of D-serine, a co-agonist of the N-methyl-D-aspartatereceptor (NMDAR), contribution of the enzyme in the NMDAR-mediated neuronal cell death process. The racemization reaction catalyzed by serine racemase may have a more protective role against apoptosis than the alpha,beta-elimination reaction Mus musculus