Literature summary for 5.1.1.18 extracted from

Zou, L.; Song, Y.; Wang, C.; Sun, J.; Wang, L.; Cheng, B.; Fan, J.
Crystal structure of maize serine racemase with pyridoxal 5-phosphate (2016), Acta Crystallogr. Sect. F, 72, 165-171 .

Cloned(Commentary)

Cloned (Comment)	Organism
sequence comparisons, cloning from leaf DNA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Zea mays

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 20 mM Tris-HCl, 100 mM NaCl, 10% glycerol, pH 8.0, with 0.001 ml of reservoir solution containing 0.2 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, 18% PEG 8000, equilibration agains 0.2 ml of reservoir solution, at 4°C, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement based on the Rattus norvegicus serine racemase, PDB ID 3hmk	Zea mays

Crystallization (Comment)

Organism

purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 20 mM Tris-HCl, 100 mM NaCl, 10% glycerol, pH 8.0, with 0.001 ml of reservoir solution containing 0.2 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, 18% PEG 8000, equilibration agains 0.2 ml of reservoir solution, at 4°C, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement based on the Rattus norvegicus serine racemase, PDB ID 3hmk

Zea mays

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
82000	-	recombinant His6-tagged enzyme, gel filtration	Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-serine	Zea mays	-	D-serine	-	r

Organism

Organism	UniProt	Comment	Textmining
Zea mays	F5CAQ9	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration	Zea mays

Reaction

Reaction	Comment	Organism	Reaction ID
L-serine = D-serine	reversible racemization and irreversible dehydration reactions are catalyzed by eukaryotic serine racemase, overview	Zea mays	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Zea mays	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine	-	Zea mays	D-serine	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 35000, recombinant His6-tagged enzyme, SDS-PAGE	Zea mays

Synonyms

Synonyms	Comment	Organism
Zm-SR	-	Zea mays
ZmSR	-	Zea mays

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	PLP, dependent on, the PLP is bound to each monomer by forming a Schiff base with Lys67	Zea mays

General Information

General Information	Comment	Organism
evolution	the enzyme from Zea mays belongs to the type II PLP-dependent enzymes and differs from the enzyme of a vancomycin-resistant bacterium	Zea mays
additional information	structural comparison with Rattus norvegicus and Schizosaccharomyces pombe serine racemases reveals a similar arrangement of active-site residues but a different orientation of the C-terminal helix, structure modeling, overview. Active site structure comparisons	Zea mays
physiological function	the enzyme is responsible for D-serine biosynthesis in vivo	Zea mays